Growth of human bone marrow in vitro

I. Svet-Moldavsky; S. C. Gulati; B. Clarkson

doi:10.1016/0309-1651(85)90102-X

Growth of human bone marrow in vitro

I. Svet-Moldavsky, S. C. Gulati, B. Clarkson

Research output: Contribution to journal › Article › peer-review

Abstract

Attempts were made to establish human bone marrow (HBM) culture. Cell proliferation was triggered by medium conditioned by acute myelocytic leukemic (AML) cells exposed to 12-O-tetradecanoyl-phorbol-13-acetate (TPA). A 6-15 fold multiplication of myelomonocytic cells was obtained in tissue culture from HBM cells. Proliferation of granulocyte-macrophage colony forming cells (CFC-GM) occurred up to 30-fold. The morphology of the cells and cytochemical markers were investigated at all stages of the culture.

Original language	English
Pages (from-to)	833-840
Number of pages	8
Journal	Cell Biology International Reports
Volume	9
Issue number	9
DOIs	https://doi.org/10.1016/0309-1651(85)90102-X
State	Published - Sep 1985
Externally published	Yes

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abstract = "Attempts were made to establish human bone marrow (HBM) culture. Cell proliferation was triggered by medium conditioned by acute myelocytic leukemic (AML) cells exposed to 12-O-tetradecanoyl-phorbol-13-acetate (TPA). A 6-15 fold multiplication of myelomonocytic cells was obtained in tissue culture from HBM cells. Proliferation of granulocyte-macrophage colony forming cells (CFC-GM) occurred up to 30-fold. The morphology of the cells and cytochemical markers were investigated at all stages of the culture.",

author = "I. Svet-Moldavsky and Gulati, {S. C.} and B. Clarkson",

year = "1985",

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AU - Svet-Moldavsky, I.

AU - Gulati, S. C.

AU - Clarkson, B.

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AB - Attempts were made to establish human bone marrow (HBM) culture. Cell proliferation was triggered by medium conditioned by acute myelocytic leukemic (AML) cells exposed to 12-O-tetradecanoyl-phorbol-13-acetate (TPA). A 6-15 fold multiplication of myelomonocytic cells was obtained in tissue culture from HBM cells. Proliferation of granulocyte-macrophage colony forming cells (CFC-GM) occurred up to 30-fold. The morphology of the cells and cytochemical markers were investigated at all stages of the culture.

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Growth of human bone marrow in vitro

Abstract

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