TY - JOUR
T1 - Growth-dependent and growth-independent translation of messengers for heterogeneous nuclear ribonucleoproteins
AU - Camacho-Vanegas, Olga
AU - Weighardt, Florian
AU - Ghigna, Claudia
AU - Amaldi, Francesco
AU - Riva, Silvano
AU - Biamonti, Giuseppe
N1 - Funding Information:
F.W. was supported by a post-doctoral fellowship from the University of Pavia; C.G. was a fellow of the CNR (AIRC); O.C.-V. was recipient of a Telethon-Italy Fellowship. This work was supported by grants form the Progetto A.I.D.S., Ministero della Sanità, Istituto Superiore della Sanità (Rome) and from the Human Capital and Mobility Program of the EC (CHRX-CT94-0677).
PY - 1997/10/1
Y1 - 1997/10/1
N2 - The hnRNP A1 transcript has a relatively short 5'-untranslated region (UTR) starting with a pyrimidine tract similar to that of mRNAs encoded by the TOP [terminal oligo(pyrimidine)] genes in vertebrates. Such genes code for ribosomal proteins and for other proteins directly or indirectly involved in the production and function of the translation apparatus. As expected from the role of the pyrimidine tract in the translational regulation of TOP mRNAs, the A1 mRNA is more efficiently loaded onto polysomes in growing than in resting cells. On the other hand, a less stringent regulation with respect to that of other TOP mRNAs is observed, partially due to the presence of multiple transcription start sites within the pyrimidine tract, where transcripts with shorter TOP sequences are less sensitive to regulation. Thus, from the point of view of structural features and translation behaviour the A1 mRNA can be included in the class of TOP genes, suggesting a possible role of A1 in translation. Interestingly, a TOP-like behaviour was observed for hnRNP I mRNA but not for hnRNP C1/C2 and A2/B1 mRNAs, indicating the existence of two classes of hnRNPs with different translational regulation.
AB - The hnRNP A1 transcript has a relatively short 5'-untranslated region (UTR) starting with a pyrimidine tract similar to that of mRNAs encoded by the TOP [terminal oligo(pyrimidine)] genes in vertebrates. Such genes code for ribosomal proteins and for other proteins directly or indirectly involved in the production and function of the translation apparatus. As expected from the role of the pyrimidine tract in the translational regulation of TOP mRNAs, the A1 mRNA is more efficiently loaded onto polysomes in growing than in resting cells. On the other hand, a less stringent regulation with respect to that of other TOP mRNAs is observed, partially due to the presence of multiple transcription start sites within the pyrimidine tract, where transcripts with shorter TOP sequences are less sensitive to regulation. Thus, from the point of view of structural features and translation behaviour the A1 mRNA can be included in the class of TOP genes, suggesting a possible role of A1 in translation. Interestingly, a TOP-like behaviour was observed for hnRNP I mRNA but not for hnRNP C1/C2 and A2/B1 mRNAs, indicating the existence of two classes of hnRNPs with different translational regulation.
UR - http://www.scopus.com/inward/record.url?scp=0030884531&partnerID=8YFLogxK
U2 - 10.1093/nar/25.19.3950
DO - 10.1093/nar/25.19.3950
M3 - Article
C2 - 9380522
AN - SCOPUS:0030884531
SN - 0305-1048
VL - 25
SP - 3950
EP - 3954
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 19
ER -