Growth and Maintenance of Glomerular Cells in Vitro

Glomeruli with viable cells were isolated from rats, dogs, and humans in large numbers by a modification of the Karkower-Greenspon method. Cellular outgrowths having the characteristics of epithelial cells were observed. A second cell type was identified within glomerular loops and remained intact as long as glomeruli were recognizable. A third cell type which resembled smooth muscle cells was seen occasionally in the outgrowth.