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Growth and characterization of high-quality protein crystals for X-ray crystallography

  • Abel Moreno
  • , Fabiano Yokaichiya
  • , Elaine Dimasi
  • , Vivian Stojanoff

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations

Abstract

Tetragonal hen egg white lysozyme is grown by the batch method in solution and gel media to study the influence of high magnetic fields on the quality of macromolecular crystals. The crystallographic quality of crystals grown in the absence and in the presence of 7- and 10-T fields are analyzed in terms of mosaicity and high-resolution X-ray imaging methods. Crystals grown by the batch method from solution showed a remarkable enhancement of the crystallographic quality, although the overall crystal quality was higher for gel-grown crystals than solution-grown crystals. The observed improvement in crystal quality can be attributed to the suppression of convective transport during the crystal growth process and the control of the nucleation kinetics by the use of a magnetic force.

Original languageEnglish
Title of host publicationInterdisciplinary Transport Phenomena Fluid, Thermal, Biological, Materials, and Space Sciences
PublisherBlackwell Publishing Inc.
Pages429-436
Number of pages8
ISBN (Print)9781573317122
DOIs
StatePublished - Apr 2009
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1161
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • 07.55.-w
  • 81.10.Dn
  • 81.20.Fw
  • Crystal growth in gels
  • Lysozyme crystals PACS 87.14.Ee
  • Magnetic fields
  • Proteins

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