Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate–protein interactions by SPR

F. Javier Muñoz; Sabine André; Hans Joachin Gabius; José V. Sinisterra; María J. Hernáiz; Robert J. Linhardt

doi:10.1039/b814171a

Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate–protein interactions by SPR

F. Javier Muñoz, Sabine André, Hans Joachin Gabius, José V. Sinisterra, María J. Hernáiz, Robert J. Linhardt

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, containing a bifunctional linker, was used to immobilize this glycoconjugate to a self assembled monolayer on a gold biosensor chip. Surface plasmon resonance (SPR) experiments demonstrated that this immobilized glycoconjugate bound to GalNAc specific lectin, Viscum album agglutinin.

Original language	English
Pages (from-to)	373-337
Number of pages	37
Journal	Green Chemistry
Volume	11
Issue number	3
DOIs	https://doi.org/10.1039/b814171a
State	Published - 9 Mar 2009
Externally published	Yes

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title = "Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate–protein interactions by SPR",

abstract = "Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, containing a bifunctional linker, was used to immobilize this glycoconjugate to a self assembled monolayer on a gold biosensor chip. Surface plasmon resonance (SPR) experiments demonstrated that this immobilized glycoconjugate bound to GalNAc specific lectin, Viscum album agglutinin.",

author = "\{Javier Mu{\~n}oz\}, F. and Sabine Andr{\'e} and Gabius, \{Hans Joachin\} and Sinisterra, \{Jos{\'e} V.\} and Hern{\'a}iz, \{Mar{\'i}a J.\} and Linhardt, \{Robert J.\}",

year = "2009",

month = mar,

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doi = "10.1039/b814171a",

language = "English",

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pages = "373--337",

journal = "Green Chemistry",

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T1 - Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate–protein interactions by SPR

AU - Javier Muñoz, F.

AU - André, Sabine

AU - Gabius, Hans Joachin

AU - Sinisterra, José V.

AU - Hernáiz, María J.

AU - Linhardt, Robert J.

PY - 2009/3/9

Y1 - 2009/3/9

N2 - Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, containing a bifunctional linker, was used to immobilize this glycoconjugate to a self assembled monolayer on a gold biosensor chip. Surface plasmon resonance (SPR) experiments demonstrated that this immobilized glycoconjugate bound to GalNAc specific lectin, Viscum album agglutinin.

AB - Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, containing a bifunctional linker, was used to immobilize this glycoconjugate to a self assembled monolayer on a gold biosensor chip. Surface plasmon resonance (SPR) experiments demonstrated that this immobilized glycoconjugate bound to GalNAc specific lectin, Viscum album agglutinin.

UR - https://www.scopus.com/pages/publications/67649418437

U2 - 10.1039/b814171a

DO - 10.1039/b814171a

M3 - Article

AN - SCOPUS:67649418437

SN - 1463-9262

VL - 11

SP - 373

EP - 337

JO - Green Chemistry

JF - Green Chemistry

IS - 3

ER -

Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate–protein interactions by SPR

Abstract

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