TY - JOUR
T1 - Graph Based Study of Allergen Cross-Reactivity of Plant Lipid Transfer Proteins (LTPs) Using Microarray in a Multicenter Study
AU - Palacín, Arantxa
AU - Gómez-Casado, Cristina
AU - Rivas, Luis A.
AU - Aguirre, Jacobo
AU - Tordesillas, Leticia
AU - Bartra, Joan
AU - Blanco, Carlos
AU - Carrillo, Teresa
AU - Cuesta-Herranz, Javier
AU - de Frutos, Consolación
AU - Álvarez-Eire, Genoveva García
AU - Fernández, Francisco J.
AU - Gamboa, Pedro
AU - Muñoz, Rosa
AU - Sánchez-Monge, Rosa
AU - Sirvent, Sofía
AU - Torres, María J.
AU - Varela-Losada, Susana
AU - Rodríguez, Rosalía
AU - Parro, Victor
AU - Blanca, Miguel
AU - Salcedo, Gabriel
AU - Díaz-Perales, Araceli
N1 - Funding Information:
Supported by Research Grants GM 13914 and HL 28481 from the National Institutes of Health.
PY - 2012/12/14
Y1 - 2012/12/14
N2 - The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
AB - The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
UR - https://www.scopus.com/pages/publications/84871267813
U2 - 10.1371/journal.pone.0050799
DO - 10.1371/journal.pone.0050799
M3 - Article
C2 - 23272072
AN - SCOPUS:84871267813
SN - 1932-6203
VL - 7
JO - PLoS ONE
JF - PLoS ONE
IS - 12
M1 - e50799
ER -