Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry

João C. Silva, Marta S. Carvalho, Ke Xia, Joaquim M.S. Cabral, Claúdia L. da Silva, Frederico C. Ferreira, Deepak Vashishth, Robert J. Linhardt

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds. Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation. However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples. In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.

Original languageEnglish
Title of host publicationCell-derived Matrices - Part A
EditorsDavid Caballero, Subhas C. Kundu, Rui Luís Reis
PublisherAcademic Press Inc.
Pages85-106
Number of pages22
ISBN (Print)9780128201725
DOIs
StatePublished - 2020
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume156
ISSN (Print)0091-679X

Keywords

  • Compositional analysis
  • Decellularized cell-derived extracellular matrices
  • Disaccharides
  • Glycosaminoglycans
  • Liquid chromatography-tandem mass spectrometrty

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