@article{b2c0d35610744e7f94e39ff00fa0bf27,
title = "Glycolipid receptor for human migration inhibitory factor: Fucose and sialic acid are important for the human monocyte response to migration inhibitory factor",
abstract = "The role of carbohydrate in the interaction of human migration inhibitory factor (MIF) with human peripheral blood monocytes was investigated by studying the effects of different exoglycosidases on the cellular response to MIF. When monocytes were pretreated with neuraminidase, an exoglycosidase specific for sialic acid, they became unresponsive to MIF. Other glycosidases, such as β-galactosidase and α-mannosidase, were inactive in this respect. The effect of neuraminidase was reversible since the response to MIF was restored to normal levels after 24 hr. In parallel studies, a glycolipid-enriched extract from U937 cells, a human macrophage-like cell line, known to enhance the monocyte response to MIF, lost this activity when treated with neuraminidase and α-l-fucosidase, but not with β-galactosidase. This suggests the importance of terminal sialic acid and fucose residues for the interaction between monocyte membrane glycolipids and MIF.",
author = "Liu, {David Y.} and Yu, {Shing Fong} and Remold, {Heinz G.} and David, {John R.}",
note = "Funding Information: {\textquoteright} This work was supported by United Public Health Service Grants AI-07685, AM-05577, and BRSG RR05669, National Science Foundation Grant PCM 8208262, Young Investigator Award AI-16021 from the National Institutes of Health, and a Milton Fund Grant. A preliminary report of this work was presented at the 66th Annual Meeting of the Federation of American Societies for Experimental Biology, New Orleans, Louisiana, held on April 15-23, 1982. 2 Requests for reprints should be addressed to Seeley G. Mudd Building, Room 504, 250 Longwood Avenue, Boston, Mass. 02 I 15. 3 Abbreviations used: MIF, migration inhibitory factor; MEM, Eagle{\textquoteright}s minimal essential medium; HBSS, Hanks{\textquoteright} balanced salt solution; M199-PS, medium 199 containing 100 U/ml penicillin and 100 &ml streptomycin; PBS, phosphate-buffered saline.",
year = "1985",
month = feb,
doi = "10.1016/0008-8749(85)90218-7",
language = "English",
volume = "90",
pages = "539--546",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",
}