Glucose-conditioned flavor preference learning requires co-activation of NMDA and dopamine D1-like receptors within the amygdala

Khalid Touzani, Richard J. Bodnar, Anthony Sclafani

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The role of amygdala (AMY) NMDA receptor signaling and its interaction with dopamine D1-like receptor signaling in glucose-mediated flavor preference learning was investigated. In Experiment 1, rats were trained with a flavor (CS+) paired with intragastric (IG) 8% glucose infusions and a different flavor (CS-) paired with IG water infusions. In the two-bottle tests (Expression), bilateral intra-AMY injections of the NMDA receptor antagonist, AP5 (0, 5 and 10. nmol/brain), did not block the CS+ preference. In Experiment 2, new rats received intra-AMY injections of either vehicle or AP5 (10. nmol), prior to training sessions with CS+/IG glucose and CS-/IG water. In the two-bottle tests without drug treatment, AP5 rats failed to prefer the CS+ flavor (50%). In Experiments 3, new rats were trained as in Experiment 2 except that, during training, half the rats received AP5 injections (5. nmol) in one side of the AMY and SCH23390 (D1-like receptor antagonist, 6. nmol), in the contralateral AMY (Drug/Drug group). The remaining rats received vehicle injections in one side of the AMY and either AP5 (5. nmol) or SCH23390 (6. nmol) in the contralateral AMY (Drug/Vehicle group). The two-bottle choice tests without drug treatment revealed that, unlike the Drug/Vehicle group (85%), the Drug/Drug group failed to prefer the CS+ flavor (50%). These results reveal an essential role for AMY NMDA receptor activation in the acquisition of flavor preference learning induced by the post-oral reinforcing properties of glucose and demonstrate that such learning is based on co-activation of NMDA and DA D1 receptors within this forebrain structure.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalNeurobiology of Learning and Memory
Volume106
DOIs
StatePublished - Nov 2013
Externally publishedYes

Keywords

  • AP5
  • Carbohydrate
  • Conditioning
  • Forebrain
  • Learning
  • Rat
  • SCH23390

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