Global Mapping of the Yeast Genetic Interaction Network

Amy Hin Yan Tong; Guillaume Lesage; Gary D. Bader; Huiming Ding; Hong Xu; Xiaofeng Xin; James Young; Gabriel F. Berriz; Renee L. Brost; Michael Chang; Yi Qun Chen; Xin Cheng; Gordon Chua; Helena Friesen; Debra S. Goldberg; Jennifer Haynes; Christine Humphries; Grace He; Shamiza Hussein; Lizhu Ke; Nevan Krogan; Zhijian Li; Joshua N. Levinson; Hong Lu; Patrice Ménard; Christella Munyana; Ainslie B. Parsons; Owen Ryan; Raffi Tonikian; Tania Roberts; Anne Marie Sdicu; Jesse Shapiro; Bilal Sheikh; Bernhard Suter; Sharyl L. Wong; Lan V. Zhang; Hongwei Zhu; Christopher G. Burd; Sean Munro; Chris Sander; Jasper Rine; Jack Greenblatt; Matthias Peter; Anthony Bretscher; Graham Bell; Frederick P. Roth; Grant W. Brown; Brenda Andrews; Howard Bussey; Charles Boone

doi:10.1126/science.1091317

Nevan Krogan, Zhijian Li, Joshua N. Levinson, Hong Lu, Patrice Ménard, Christella Munyana, Ainslie B. Parsons, Owen Ryan, Raffi Tonikian, Tania Roberts, Anne Marie Sdicu, Jesse Shapiro, Bilal Sheikh, Bernhard Suter, Sharyl L. Wong, Lan V. Zhang, Hongwei Zhu, Christopher G. Burd, Sean Munro, Chris Sander, Jasper Rine, Jack Greenblatt, Matthias Peter, Anthony Bretscher, Graham Bell, Frederick P. Roth, Grant W. Brown, Brenda Andrews, Howard Bussey, Charles Boone

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1744 Scopus citations

Abstract

A genetic interaction network containing ∼1000 genes and ∼4000 interactions was mapped by crossing mutations in 132 different query genes into a set of ∼4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

Original language	English
Pages (from-to)	808-813
Number of pages	6
Journal	Science
Volume	303
Issue number	5659
DOIs	https://doi.org/10.1126/science.1091317
State	Published - 6 Feb 2004
Externally published	Yes

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Tong, A. H. Y., Lesage, G., Bader, G. D., Ding, H., Xu, H., Xin, X., Young, J., Berriz, G. F., Brost, R. L., Chang, M., Chen, Y. Q., Cheng, X., Chua, G., Friesen, H., Goldberg, D. S., Haynes, J., Humphries, C., He, G., Hussein, S., ... Boone, C. (2004). Global Mapping of the Yeast Genetic Interaction Network. Science, 303(5659), 808-813. https://doi.org/10.1126/science.1091317

@article{8fabcd01266e4985b3883db5c2c6d81f,

title = "Global Mapping of the Yeast Genetic Interaction Network",

abstract = "A genetic interaction network containing ∼1000 genes and ∼4000 interactions was mapped by crossing mutations in 132 different query genes into a set of ∼4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.",

author = "Tong, \{Amy Hin Yan\} and Guillaume Lesage and Bader, \{Gary D.\} and Huiming Ding and Hong Xu and Xiaofeng Xin and James Young and Berriz, \{Gabriel F.\} and Brost, \{Renee L.\} and Michael Chang and Chen, \{Yi Qun\} and Xin Cheng and Gordon Chua and Helena Friesen and Goldberg, \{Debra S.\} and Jennifer Haynes and Christine Humphries and Grace He and Shamiza Hussein and Lizhu Ke and Nevan Krogan and Zhijian Li and Levinson, \{Joshua N.\} and Hong Lu and Patrice M{\'e}nard and Christella Munyana and Parsons, \{Ainslie B.\} and Owen Ryan and Raffi Tonikian and Tania Roberts and Sdicu, \{Anne Marie\} and Jesse Shapiro and Bilal Sheikh and Bernhard Suter and Wong, \{Sharyl L.\} and Zhang, \{Lan V.\} and Hongwei Zhu and Burd, \{Christopher G.\} and Sean Munro and Chris Sander and Jasper Rine and Jack Greenblatt and Matthias Peter and Anthony Bretscher and Graham Bell and Roth, \{Frederick P.\} and Brown, \{Grant W.\} and Brenda Andrews and Howard Bussey and Charles Boone",

year = "2004",

month = feb,

day = "6",

doi = "10.1126/science.1091317",

language = "English",

volume = "303",

pages = "808--813",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5659",

}

Tong, AHY, Lesage, G, Bader, GD, Ding, H, Xu, H, Xin, X, Young, J, Berriz, GF, Brost, RL, Chang, M, Chen, YQ, Cheng, X, Chua, G, Friesen, H, Goldberg, DS, Haynes, J, Humphries, C, He, G, Hussein, S, Ke, L, Krogan, N, Li, Z, Levinson, JN, Lu, H, Ménard, P, Munyana, C, Parsons, AB, Ryan, O, Tonikian, R, Roberts, T, Sdicu, AM, Shapiro, J, Sheikh, B, Suter, B, Wong, SL, Zhang, LV, Zhu, H, Burd, CG, Munro, S, Sander, C, Rine, J, Greenblatt, J, Peter, M, Bretscher, A, Bell, G, Roth, FP, Brown, GW, Andrews, B, Bussey, H & Boone, C 2004, 'Global Mapping of the Yeast Genetic Interaction Network', Science, vol. 303, no. 5659, pp. 808-813. https://doi.org/10.1126/science.1091317

TY - JOUR

T1 - Global Mapping of the Yeast Genetic Interaction Network

AU - Tong, Amy Hin Yan

AU - Lesage, Guillaume

AU - Bader, Gary D.

AU - Ding, Huiming

AU - Xu, Hong

AU - Xin, Xiaofeng

AU - Young, James

AU - Berriz, Gabriel F.

AU - Brost, Renee L.

AU - Chang, Michael

AU - Chen, Yi Qun

AU - Cheng, Xin

AU - Chua, Gordon

AU - Friesen, Helena

AU - Goldberg, Debra S.

AU - Haynes, Jennifer

AU - Humphries, Christine

AU - He, Grace

AU - Hussein, Shamiza

AU - Ke, Lizhu

AU - Krogan, Nevan

AU - Li, Zhijian

AU - Levinson, Joshua N.

AU - Lu, Hong

AU - Ménard, Patrice

AU - Munyana, Christella

AU - Parsons, Ainslie B.

AU - Ryan, Owen

AU - Tonikian, Raffi

AU - Roberts, Tania

AU - Sdicu, Anne Marie

AU - Shapiro, Jesse

AU - Sheikh, Bilal

AU - Suter, Bernhard

AU - Wong, Sharyl L.

AU - Zhang, Lan V.

AU - Zhu, Hongwei

AU - Burd, Christopher G.

AU - Munro, Sean

AU - Sander, Chris

AU - Rine, Jasper

AU - Greenblatt, Jack

AU - Peter, Matthias

AU - Bretscher, Anthony

AU - Bell, Graham

AU - Roth, Frederick P.

AU - Brown, Grant W.

AU - Andrews, Brenda

AU - Bussey, Howard

AU - Boone, Charles

PY - 2004/2/6

Y1 - 2004/2/6

N2 - A genetic interaction network containing ∼1000 genes and ∼4000 interactions was mapped by crossing mutations in 132 different query genes into a set of ∼4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

AB - A genetic interaction network containing ∼1000 genes and ∼4000 interactions was mapped by crossing mutations in 132 different query genes into a set of ∼4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

UR - https://www.scopus.com/pages/publications/10744230485

U2 - 10.1126/science.1091317

DO - 10.1126/science.1091317

M3 - Article

C2 - 14764870

AN - SCOPUS:10744230485

SN - 0036-8075

VL - 303

SP - 808

EP - 813

JO - Science

JF - Science

IS - 5659

ER -

Global Mapping of the Yeast Genetic Interaction Network

Abstract

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