Getting Started with LINCS Datasets and Tools

Zhuorui Xie, Eryk Kropiwnicki, Megan L. Wojciechowicz, Kathleen M. Jagodnik, Ingrid Shu, Allison Bailey, Daniel J.B. Clarke, Minji Jeon, John Erol Evangelista, Maxim V. Kuleshov, Alexander Lachmann, Abhijna A. Parigi, Jose M. Sanchez, Sherry L. Jenkins, Avi Ma'ayan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The Library of Integrated Network-based Cellular Signatures (LINCS) was an NIH Common Fund program that aimed to expand our knowledge about human cellular responses to chemical, genetic, and microenvironment perturbations. Responses to perturbations were measured by transcriptomics, proteomics, cellular imaging, and other high content assays. The second phase of the LINCS program, which lasted 7 years, involved the engagement of six data and signature generation centers (DSGCs) and one data coordination and integration center (DCIC). The DSGCs and the DCIC developed several digital resources, including tools, databases, and workflows that aim to facilitate the use of the LINCS data and integrate this data with other publicly available data. The digital resources developed by the DSGCs and the DCIC can be used to gain new biological and pharmacological insights that can lead to the development of novel therapeutics. This protocol provides step-by-step instructions for processing the LINCS data into signatures, and utilizing the digital resources developed by the LINCS consortia for hypothesis generation and knowledge discovery.

Original languageEnglish
Article numbere487
JournalCurrent Protocols
Volume2
Issue number7
DOIs
StatePublished - Jul 2022

Keywords

  • bioinformatics
  • disease
  • drug discovery
  • gene sets visualization
  • signature analysis
  • web application

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