Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme

Junjie Yang; Yimeng Kong; Sheng Yang

doi:10.1186/s40643-014-0030-8

Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme

Junjie Yang, Yimeng Kong, Sheng Yang

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Background: Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains. Thus, an efficient method for genotyping of the C. glutamicum is heavily called. Results: Multi-locus sequence typing (MLST) is currently the most popular molecular typing technique. But currently this method is not available for C. glutamicum. In this study, a MLST scheme was established based on sequences of seven housekeeping genes, for genotyping of C. glutamicum. The MLST method performed an efficient discrimination of 17 strains and helps to understand the population structure of this bacterium. Conclusions: This work has expanded the MLST method to C. glutamicum and developed an efficient technique to discriminate strains of uncertain origin.

Original language	English
Article number	1
Journal	Bioresources and Bioprocessing
Volume	2
Issue number	1
DOIs	https://doi.org/10.1186/s40643-014-0030-8
State	Published - 1 Dec 2015
Externally published	Yes

Keywords

16S rDNA
Amino acid producing
Corynebacterium crenatum
Corynebacterium glutamicum
Multi-locus sequence typing

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10.1186/s40643-014-0030-8

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title = "Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme",

abstract = "Background: Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains. Thus, an efficient method for genotyping of the C. glutamicum is heavily called. Results: Multi-locus sequence typing (MLST) is currently the most popular molecular typing technique. But currently this method is not available for C. glutamicum. In this study, a MLST scheme was established based on sequences of seven housekeeping genes, for genotyping of C. glutamicum. The MLST method performed an efficient discrimination of 17 strains and helps to understand the population structure of this bacterium. Conclusions: This work has expanded the MLST method to C. glutamicum and developed an efficient technique to discriminate strains of uncertain origin.",

keywords = "16S rDNA, Amino acid producing, Corynebacterium crenatum, Corynebacterium glutamicum, Multi-locus sequence typing",

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AU - Yang, Junjie

AU - Kong, Yimeng

AU - Yang, Sheng

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Background: Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains. Thus, an efficient method for genotyping of the C. glutamicum is heavily called. Results: Multi-locus sequence typing (MLST) is currently the most popular molecular typing technique. But currently this method is not available for C. glutamicum. In this study, a MLST scheme was established based on sequences of seven housekeeping genes, for genotyping of C. glutamicum. The MLST method performed an efficient discrimination of 17 strains and helps to understand the population structure of this bacterium. Conclusions: This work has expanded the MLST method to C. glutamicum and developed an efficient technique to discriminate strains of uncertain origin.

AB - Background: Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains. Thus, an efficient method for genotyping of the C. glutamicum is heavily called. Results: Multi-locus sequence typing (MLST) is currently the most popular molecular typing technique. But currently this method is not available for C. glutamicum. In this study, a MLST scheme was established based on sequences of seven housekeeping genes, for genotyping of C. glutamicum. The MLST method performed an efficient discrimination of 17 strains and helps to understand the population structure of this bacterium. Conclusions: This work has expanded the MLST method to C. glutamicum and developed an efficient technique to discriminate strains of uncertain origin.

KW - 16S rDNA

KW - Amino acid producing

KW - Corynebacterium crenatum

KW - Corynebacterium glutamicum

KW - Multi-locus sequence typing

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DO - 10.1186/s40643-014-0030-8

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Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme

Abstract

Keywords

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