Genomics, Proteomics, and Neurology

Lorelei D. Shoemaker, Daniel H. Geschwind

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


This chapter focuses on how one can assess the functional consequences arising from the genome or the interactions of the genome and the environment in a high throughput manner at the transcriptome and proteome level. To understand the role of genes in development, functioning, and diseases of the nervous system, it is needed to measure the dynamic patterns of gene expression within specific brain regions and cell types. To perform this task efficiently requires methods allowing study of the expression patterns of large number of genes and their regulation at the translational and posttranslational. Microarrays permit tour de force monitoring of the entire transcriptome of a given neuronal or glial cell or brain region. Rapidly evolving proteomics techniques, such as mass spectrometry coupled to two-dimensional gel electrophoresis (2DGE) permit similar assessment of the proteome. Microarrays and proteomic techniques provide a striking improvement in power and throughput over conventional techniques such as reverse transcription-polymerase chain reaction (RT-PCR), and Northern or Western blotting, which allow the simultaneous assessment of only single or small groups of genes and proteins.

Original languageEnglish
Title of host publicationFrom Neuroscience to Neurology
Subtitle of host publicationNeuroscience, Molecular Medicine, and the Therapeutic Transformation of Neurology
Number of pages20
ISBN (Print)9780127389035
StatePublished - 18 Oct 2004
Externally publishedYes


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