Genomic structure of and a cardiac promoter for the mousecarnitine palmitoyltransferase II gene

Bruce D. Gelb

doi:10.1016/S0888-7543(05)80368-4

Genomic structure of and a cardiac promoter for the mousecarnitine palmitoyltransferase II gene

Bruce D. Gelb

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The mouse carnitine palmitoyltransferase II gene hasbeen isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5′ rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver.

Original language	English
Pages (from-to)	651-655
Number of pages	5
Journal	Genomics
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/S0888-7543(05)80368-4
State	Published - Dec 1993
Externally published	Yes

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title = "Genomic structure of and a cardiac promoter for the mousecarnitine palmitoyltransferase II gene",

abstract = "The mouse carnitine palmitoyltransferase II gene hasbeen isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5′ rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver.",

author = "Gelb, \{Bruce D.\}",

note = "Funding Information: This work was supported by a Young Pediatric Investigatorship to B.D.G. from the Mount Sinai Children's He\textasciitilde{}alth Research Center award (1 P30 HD 28822-02) from the National Institute of Children's Health and Development. The author thanks J. Zhang for her expert technical assistance, Y. A. Ioannou and D. F. Bishop for their helpful advice, and R. J. Desnick for his support and thoughtful reading of the manuscript.",

year = "1993",

month = dec,

doi = "10.1016/S0888-7543(05)80368-4",

language = "English",

volume = "18",

pages = "651--655",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

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T1 - Genomic structure of and a cardiac promoter for the mousecarnitine palmitoyltransferase II gene

AU - Gelb, Bruce D.

N1 - Funding Information: This work was supported by a Young Pediatric Investigatorship to B.D.G. from the Mount Sinai Children's He~alth Research Center award (1 P30 HD 28822-02) from the National Institute of Children's Health and Development. The author thanks J. Zhang for her expert technical assistance, Y. A. Ioannou and D. F. Bishop for their helpful advice, and R. J. Desnick for his support and thoughtful reading of the manuscript.

PY - 1993/12

Y1 - 1993/12

N2 - The mouse carnitine palmitoyltransferase II gene hasbeen isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5′ rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver.

AB - The mouse carnitine palmitoyltransferase II gene hasbeen isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5′ rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver.

UR - https://www.scopus.com/pages/publications/0027770698

U2 - 10.1016/S0888-7543(05)80368-4

DO - 10.1016/S0888-7543(05)80368-4

M3 - Article

C2 - 8307575

AN - SCOPUS:0027770698

SN - 0888-7543

VL - 18

SP - 651

EP - 655

JO - Genomics

JF - Genomics

IS - 3

ER -

Genomic structure of and a cardiac promoter for the mousecarnitine palmitoyltransferase II gene

Abstract

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