TY - JOUR
T1 - Genomic organization and chromosomal localization of the human histone deacetylase 3 gene
AU - Mahlknecht, Ulrich
AU - Emiliani, Stephane
AU - Najfeld, Vesna
AU - Young, Steven
AU - Verdin, Eric
N1 - Funding Information:
The authors thank Richard Bucala and Kirk Manogue for critically reading the manuscript, Leslie Goodman and Craig Gawel for stimulating discussions, Stephen Ordway and Gary Howard for editorial assistance, and Neile Shea for graphics. This work was partly supported by the German National Science Foundation (Deutsche For-schungsgemeinschaft, MA 2057/1-1) and institutional funds from the Picower Institute for Medical Research and the Gladstone Institute of Virology and Immunology.
PY - 1999/3/1
Y1 - 1999/3/1
N2 - Reversible acetylation of histone proteins plays a critical role in transcriptional regulation, cell cycle progression, and developmental events. The steady state of histone acetylation is controlled by the enzymatic activities of multiple histone acetyltransferases and histone deacetylases (HDACs). Three distinct human HDACs are homologous to RPD3, a yeast transcriptional regulator. We have isolated and sequenced a genomic clone for the human HDAC3 gene. This is a single-copy gene spanning a region of at least 13 kb. Determination of the intron-exon splice junctions established that the gene is encoded by 15 exons ranging in size from 56 to 657 bp. Fluorescence in situ hybridization studies localized this gene to 5q31. Double-target experiments in which both HDAC3 and the early-growth response 1 gene (EGR1), which is localized in the 5q31.2 region, were used as probes showed that the HDAC3 gene lies in region 5q31.3, immediately distal to EGR1 with respect to the centromere.
AB - Reversible acetylation of histone proteins plays a critical role in transcriptional regulation, cell cycle progression, and developmental events. The steady state of histone acetylation is controlled by the enzymatic activities of multiple histone acetyltransferases and histone deacetylases (HDACs). Three distinct human HDACs are homologous to RPD3, a yeast transcriptional regulator. We have isolated and sequenced a genomic clone for the human HDAC3 gene. This is a single-copy gene spanning a region of at least 13 kb. Determination of the intron-exon splice junctions established that the gene is encoded by 15 exons ranging in size from 56 to 657 bp. Fluorescence in situ hybridization studies localized this gene to 5q31. Double-target experiments in which both HDAC3 and the early-growth response 1 gene (EGR1), which is localized in the 5q31.2 region, were used as probes showed that the HDAC3 gene lies in region 5q31.3, immediately distal to EGR1 with respect to the centromere.
UR - https://www.scopus.com/pages/publications/0033105705
U2 - 10.1006/geno.1998.5645
DO - 10.1006/geno.1998.5645
M3 - Article
C2 - 10051405
AN - SCOPUS:0033105705
SN - 0888-7543
VL - 56
SP - 197
EP - 202
JO - Genomics
JF - Genomics
IS - 2
ER -