Genomic imprinting defect in Zfp57 mutant iPS cell lines

Carol M. McDonald, Lizhi Liu, Lijuan Xiao, Christoph Schaniel, Xiajun Li

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


ZFP57 maintains genomic imprinting in mouse embryos and ES cells. To test its roles during iPS reprogramming, we derived iPS clones by utilizing retroviral infection to express reprogramming factors in mouse MEF cells. After analyzing four imprinted regions, we found that parentally derived DNA methylation imprint was largely maintained in the iPS clones with Zfp57 but missing in those without maternal or zygotic Zfp57. Intriguingly, DNA methylation imprint was lost at the Peg1 and Peg3 but retained at the Snrpn and Dlk1-Dio3 imprinted regions in the iPS clones without zygotic Zfp57. This finding will be pursued in future studies.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalStem Cell Research
Issue number2
StatePublished - 1 Mar 2016


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