Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity

Satoshi Ikegame, Shannon M. Beaty, Christian Stevens, Sohui T. Won, Arnold Park, David Sachs, Patrick Hong, Benhur Lee, Patricia A. Thibault

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The antigenic and genomic stability of paramyxoviruses remains a mystery. Here, we evaluate the genetic plasticity of Sendai virus (SeV) and mumps virus (MuV), sialic acid-using paramyxoviruses that infect mammals from two Paramyxoviridae subfamilies (Orthoparamyxovirinae and Rubulavirinae). We performed saturating whole-genome transposon insertional mutagenesis, and identified important commonalities: disordered regions in the N and P genes near the 3' genomic end were more tolerant to insertional disruptions; but the envelope glycoproteins were not, highlighting structural constraints that contribute to the restricted antigenic drift in paramyxoviruses. Nonetheless, when we applied our strategy to a fusion-defective Newcastle disease virus (Avulavirinae subfamily), we could select for Frevertants and other insertants in the 5′ end of the genome. Our genome-wide interrogation of representative paramyxovirus genomes from all three Paramyxoviridae subfamilies provides a family-wide context in which to explore specific variations within and among paramyxovirus genera and species.

Original languageEnglish
Article numbere1008877
JournalPLoS Pathogens
Issue number10
StatePublished - 9 Oct 2020


Dive into the research topics of 'Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity'. Together they form a unique fingerprint.

Cite this