TY - JOUR
T1 - Genetic refinement and physical mapping of a chromosome 16q candidate region for inflammatory bowel disease
AU - Zouali, Habib
AU - Chamaillard, Mathias
AU - Lesage, Suzane
AU - Cézard, Jean Pierre
AU - Colombel, Jean Frédéric
AU - Belaiche, Jacques
AU - Almer, Sven
AU - Tysk, Curt
AU - Montague, Sean
AU - Gassull, Miquel
AU - Christensen, Steen
AU - Finkel, Yigael
AU - Gower-Rousseau, Corine
AU - Modigliani, Robert
AU - Macry, Jeanne
AU - Selinger-Leneman, Hana
AU - Thomas, Gilles
AU - Hugot, Jean Pierre
N1 - Funding Information:
In 1995, under a grant from the National Aeronautics and Space Administration (NASA), four core volumes were selected from the 11 to initiate the RSCC program. These four volumes represent the fundamental topics that were needed in remote sensing education across disciplines. From this foundation, it was envisioned that other volumes would be added in time. The four fundamental volumes chosen include: Volume 1: modules contain the materials needed to fully understand the topic being described. Images, graphs, and charts provide useful information to help the user better comprehend the materials. Exercise modules are available that allow the user to download raw satellite data to their image processing system and complete an exercise using the raw satellite data. Advances in Internet technology facilitate instant access, or hot links, to related materials, references, and other appropriate Web sites (Foresman, et al., 1997).
PY - 2001
Y1 - 2001
N2 - Crohn's disease (CD) is a complex genetic disorder for which a susceptibility gene, IBD1, has been mapped within the pericentromeric region of chromosome 16. In order to refine the location of IBD1, 77 multiplex CD families were genotyped for 26 microsatellite markers evenly spaced by approximately 1 cM. Nonparametric linkage analyses exhibited a maximum NPL score of 3.49 (P=2.37 × 10-4) in a region centred by markers D16S3136, D16S3117 and D16S770. Simulation studies showed that the probability for IBD1 to be located in a 5 cM region around these markers was 70%. A 2.5 Mb YAC and BAC contig map spanning this genetic region on chromosome band 16q12 was built. TDT analyses demonstrated suggestive association between the 207 bp allele of D16S3136 (P<0.05) and a new biallellic marker hb27g11f-end (P=0.01). These markers were located in the hb27g11 and hb87b10 BAC clones from the contig. Taken together, the present results provide a crucial preliminary step before an exhaustive linkage disequilibrium mapping of putatively transcribed regions to identify IBD1.
AB - Crohn's disease (CD) is a complex genetic disorder for which a susceptibility gene, IBD1, has been mapped within the pericentromeric region of chromosome 16. In order to refine the location of IBD1, 77 multiplex CD families were genotyped for 26 microsatellite markers evenly spaced by approximately 1 cM. Nonparametric linkage analyses exhibited a maximum NPL score of 3.49 (P=2.37 × 10-4) in a region centred by markers D16S3136, D16S3117 and D16S770. Simulation studies showed that the probability for IBD1 to be located in a 5 cM region around these markers was 70%. A 2.5 Mb YAC and BAC contig map spanning this genetic region on chromosome band 16q12 was built. TDT analyses demonstrated suggestive association between the 207 bp allele of D16S3136 (P<0.05) and a new biallellic marker hb27g11f-end (P=0.01). These markers were located in the hb27g11 and hb87b10 BAC clones from the contig. Taken together, the present results provide a crucial preliminary step before an exhaustive linkage disequilibrium mapping of putatively transcribed regions to identify IBD1.
KW - Contig map
KW - Crohn's disease
KW - Fine mapping
KW - Linkage disequilibrium
KW - Positional cloning
UR - http://www.scopus.com/inward/record.url?scp=0034752121&partnerID=8YFLogxK
U2 - 10.1038/sj.ejhg.5200710
DO - 10.1038/sj.ejhg.5200710
M3 - Article
C2 - 11781683
AN - SCOPUS:0034752121
SN - 1018-4813
VL - 9
SP - 731
EP - 742
JO - European Journal of Human Genetics
JF - European Journal of Human Genetics
IS - 10
ER -