TY - JOUR
T1 - Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan
AU - Kuroda, Moegi
AU - Masuda, Tsuneyuki
AU - Ito, Mika
AU - Naoi, Yuki
AU - Doan, Yen Hai
AU - Haga, Kei
AU - Tsuchiaka, Shinobu
AU - Kishimoto, Mai
AU - Sano, Kaori
AU - Omatsu, Tsutomu
AU - Katayama, Yukie
AU - Oba, Mami
AU - Aoki, Hiroshi
AU - Ichimaru, Toru
AU - Sunaga, Fujiko
AU - Mukono, Itsuro
AU - Yamasato, Hiroshi
AU - Shirai, Junsuke
AU - Katayama, Kazuhiko
AU - Mizutani, Tetsuya
AU - Oka, Tomoichiro
AU - Nagai, Makoto
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/11
Y1 - 2017/11
N2 - Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (> 6000 nucleotide: nt) and three partial sequences (2429 nt, 4364 nt, and 4419 nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach. Phylogenetic analysis of the complete VP1 amino acid sequence (aa) revealed that 29 porcine SaVs were classified into seven genogroups; GIII (11 strains), GV (1 strain), GVI (3 strains), GVII (6 strains), GVIII (1 strain), GX (3 strains), and GXI (4 strains). This manuscript presents the first nearly complete genome sequences of GX and GXI, and demonstrates novel intergenogroup recombination events.
AB - Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (> 6000 nucleotide: nt) and three partial sequences (2429 nt, 4364 nt, and 4419 nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach. Phylogenetic analysis of the complete VP1 amino acid sequence (aa) revealed that 29 porcine SaVs were classified into seven genogroups; GIII (11 strains), GV (1 strain), GVI (3 strains), GVII (6 strains), GVIII (1 strain), GX (3 strains), and GXI (4 strains). This manuscript presents the first nearly complete genome sequences of GX and GXI, and demonstrates novel intergenogroup recombination events.
KW - Genogroup
KW - Japan
KW - Pig
KW - Recombination
KW - Sapovirus
UR - https://www.scopus.com/pages/publications/85029725560
U2 - 10.1016/j.meegid.2017.09.013
DO - 10.1016/j.meegid.2017.09.013
M3 - Article
C2 - 28923281
AN - SCOPUS:85029725560
SN - 1567-1348
VL - 55
SP - 209
EP - 217
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -