Genetic basis of emerging vancomycin, linezolid, and daptomycin heteroresistance in a case of persistent Enterococcus faecium bacteremia

Kieran I. Chacko, Mitchell J. Sullivan, Colleen Beckford, Deena R. Altman, Brianne Ciferri, Theodore R. Pak, Robert Sebra, Andrew Kasarskis, Camille L. Hamula, Harm Van Bakel

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Whole-genome sequencing was used to examine a persistent Enterococcus faecium bacteremia that acquired heteroresistance to three antibiotics in response to prolonged multidrug therapy. A comparison of the complete genomes before and after each change revealed the emergence of known resistance determinants for vancomycin and linezolid and suggested that a novel mutation in fabF, encoding a fatty acid synthase, was responsible for daptomycin nonsusceptibility. Plasmid recombination contributed to the progressive loss of vancomycin resistance after withdrawal of the drug.

Original languageEnglish
Article numbere02007-17
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number4
DOIs
StatePublished - Apr 2018

Keywords

  • Daptomycin
  • E. faecium
  • FabF
  • Heteroresistance
  • Linezolid
  • VRE
  • Vancomycin

Fingerprint

Dive into the research topics of 'Genetic basis of emerging vancomycin, linezolid, and daptomycin heteroresistance in a case of persistent Enterococcus faecium bacteremia'. Together they form a unique fingerprint.

Cite this