Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse

Jeffrey Barminko, Andrei M. Vacaru, Margaret H. Baron

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Hematopoiesis in the mouse and other mammals occurs in several waves and arises from distinct anatomic sites. Transgenic mice expressing fluorescent reporter proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to more restricted progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene, survey available fluorescent probes, and describe methods for confirming and analyzing transgene expression in the hematopoietic tissues of the embryo, fetus, and postnatal/adult animal.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-182
Number of pages30
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2224
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Fluorescent reporter
  • Green fluorescent protein
  • Hematopoiesis
  • Knock-in
  • Transgenic mice

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