Efforts by a number of influenza research groups have been pivotal in the development and improvement of influenza A virus reverse genetics. Originally established in 19991,2 plasmid-based reverse genetic techniques to generate recombinant viruses have revolutionized the influenza research field because specific questions have been answered by genetically engineered, infectious, recombinant influenza viruses. Such studies include virus replication, function of viral proteins, the contribution of specific mutations in viral proteins in viral replication and/or pathogenesis and, also, viral vectors using recombinant influenza viruses expressing foreign proteins3.

Original languageEnglish
Article numbere2057
JournalJournal of Visualized Experiments
Issue number42
StatePublished - Aug 2010


  • HA assay
  • Influenza viruses
  • Issue 42
  • Microbiology
  • Plasmid transfection
  • Recombinant virus
  • Reverse genetics techniques


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