@inbook{03bea2276dd2475a90641623a7b20476,
title = "Generation of mouse bone marrow-derived macrophages using tumor coculture assays to mimic the tumor microenvironment",
abstract = "Macrophages are one of the key immune cells within the tumor microenvironment that encourage the growth of tumors at the primary site as well as contributing to all parts of the metastatic cascade. Although it is possible to isolate macrophages directly from the tumor, this can be a laborious process and due to their plasticity, it is not possible to maintain their in vivo phenotype in vitro. For this reason, differentiating macrophages from bone marrow is an attractive alternative. Here we present robust methods to study in vitro derived macrophages including (i) the isolation and generation of macrophages from bone marrow, (ii) differentiation/characterization of classically activated, alternatively activated and tumor-conditioned macrophages, as well as (iii) in vitro co-culturing assays for tumor cell-macrophage interaction/transmigration.",
keywords = "Bone marrow, Macrophages, Transmigration, Tumor-macrophage coculture",
author = "Esra G{\"u}{\c c} and Demi Brownlie and Carolina Rodriguez-Tirado and Takanori Kitamura and Pollard, {Jeffrey W.}",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
doi = "10.1016/bs.mie.2019.11.014",
language = "English",
isbn = "9780128186756",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "91--111",
editor = "Lorenzo Galluzzi and Nils-Petter Rudqvist",
booktitle = "Tumor Immunology and Immunotherapy - Cellular Methods Part B",
address = "United States",
}