Generation of a tamoxifen inducible Tnnt2MerCreMer knock-in mouse model for cardiac studies

Jianyun Yan, Nishat Sultana, Lu Zhang, David S. Park, Akshay Shekhar, Jun Hu, Lei Bu, Chen Leng Cai

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Tnnt2, encoding thin-filament sarcomeric protein cardiac troponin T, plays critical roles in heart development and function in mammals. To develop an inducible genetic deletion strategy in myocardial cells, we generated a new Tnnt2:MerCreMer (Tnnt2MerCreMer/+) knock-in mouse. Rosa26 reporter lines were used to examine the specificity and efficiency of the inducible Cre recombinase. We found that Cre was specifically and robustly expressed in the cardiomyocytes at embryonic and adult stages following tamoxifen induction. The knock-in allele on Tnnt2 locus does not impact cardiac function. These results suggest that this new Tnnt2MerCreMer/+ mouse could be applied towards the temporal genetic deletion of genes of interests in cardiomyocytes with Cre-LoxP technology. The Tnnt2MerCreMer/+ mouse model also provides a useful tool to trace myocardial lineage during development and repair after cardiac injury.

Original languageEnglish
Pages (from-to)377-386
Number of pages10
JournalGenesis
Volume53
Issue number6
DOIs
StatePublished - 1 Jun 2015

Keywords

  • Cardiomyocyte
  • Heart
  • MerCreMer
  • Tamoxifen
  • Tnnt2

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