Generation and identification of renal tubular epithelial cell C/EBPα conditional knockout mice

Ji Ying; Jian Liu; Li Wen Zhang; Fang Zhong; Fang Fang Zhou; Wei Ming Wang

doi:10.3969/j.issn.1674-8115.2016.10.001

Generation and identification of renal tubular epithelial cell C/EBPα conditional knockout mice

Ji Ying, Jian Liu, Li Wen Zhang, Fang Zhong, Fang Fang Zhou, Wei Ming Wang

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To generate renal tubular epithelial cell C/EBPα conditional knockout mice, perform phenotypic identification, and lay a foundation for further study on the role of C/EBPα in renal interstitial fibrosis. Methods: C/EBPα^flox/flox and pepck-Cre⁺ transgenic mice were bred and hybridized. The first generation offspring were mated to reproduce the second generation. Mice with C/EBPα^flox/flox. pepck-Cre⁺ genotype (C/EBPα^-/- mice) were identified using PCR. The mRNA and protein expressions of C/EBPα in renal tissues were measured using RT-qPCR and immunohistochemistry staining. The morphologic changes in renal tissues of C/EBPα^-/- mice and wide type (WT) mice aged 6-8 weeks were observed using H-E staining and Masson staining. Results: The genotype of the second generation offspring of two types of transgenic mice accorded with the Mendelian laws and C/EBPα^-/- mice were generated. C/EBPα^-/- mice had lower mRNA and protein expressions of C/EBPα in renal tissues and no significant renal morphologic changes compared with WT mice. Conclusion: Cre-loxp system can successfully knock out the C/EBPα gene in mouse renal tubular epithelial cells. C/EBPα^-/- mice have no significant renal morphologic changes in baseline condition.

Original language	English
Pages (from-to)	1403-1407
Number of pages	5
Journal	Journal of Shanghai Jiaotong University (Medical Science)
Volume	36
Issue number	10
DOIs	https://doi.org/10.3969/j.issn.1674-8115.2016.10.001
State	Published - 28 Oct 2016
Externally published	Yes

Keywords

C/EBPα
Conditional knockout
Cre-loxp system
Renal tubular epithelial cells

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10.3969/j.issn.1674-8115.2016.10.001

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title = "Generation and identification of renal tubular epithelial cell C/EBPα conditional knockout mice",

abstract = "Objective: To generate renal tubular epithelial cell C/EBPα conditional knockout mice, perform phenotypic identification, and lay a foundation for further study on the role of C/EBPα in renal interstitial fibrosis. Methods: C/EBPαflox/flox and pepck-Cre+ transgenic mice were bred and hybridized. The first generation offspring were mated to reproduce the second generation. Mice with C/EBPαflox/flox. pepck-Cre+ genotype (C/EBPα-/- mice) were identified using PCR. The mRNA and protein expressions of C/EBPα in renal tissues were measured using RT-qPCR and immunohistochemistry staining. The morphologic changes in renal tissues of C/EBPα-/- mice and wide type (WT) mice aged 6-8 weeks were observed using H-E staining and Masson staining. Results: The genotype of the second generation offspring of two types of transgenic mice accorded with the Mendelian laws and C/EBPα-/- mice were generated. C/EBPα-/- mice had lower mRNA and protein expressions of C/EBPα in renal tissues and no significant renal morphologic changes compared with WT mice. Conclusion: Cre-loxp system can successfully knock out the C/EBPα gene in mouse renal tubular epithelial cells. C/EBPα-/- mice have no significant renal morphologic changes in baseline condition.",

keywords = "C/EBPα, Conditional knockout, Cre-loxp system, Renal tubular epithelial cells",

author = "Ji Ying and Jian Liu and Zhang, {Li Wen} and Fang Zhong and Zhou, {Fang Fang} and Wang, {Wei Ming}",

year = "2016",

month = oct,

day = "28",

doi = "10.3969/j.issn.1674-8115.2016.10.001",

language = "English",

volume = "36",

pages = "1403--1407",

journal = "Journal of Shanghai Jiaotong University (Medical Science)",

issn = "1674-8115",

publisher = "Editorial Department of Journal of Shanghai Second Medical University",

number = "10",

}

TY - JOUR

T1 - Generation and identification of renal tubular epithelial cell C/EBPα conditional knockout mice

AU - Ying, Ji

AU - Liu, Jian

AU - Zhang, Li Wen

AU - Zhong, Fang

AU - Zhou, Fang Fang

AU - Wang, Wei Ming

PY - 2016/10/28

Y1 - 2016/10/28

N2 - Objective: To generate renal tubular epithelial cell C/EBPα conditional knockout mice, perform phenotypic identification, and lay a foundation for further study on the role of C/EBPα in renal interstitial fibrosis. Methods: C/EBPαflox/flox and pepck-Cre+ transgenic mice were bred and hybridized. The first generation offspring were mated to reproduce the second generation. Mice with C/EBPαflox/flox. pepck-Cre+ genotype (C/EBPα-/- mice) were identified using PCR. The mRNA and protein expressions of C/EBPα in renal tissues were measured using RT-qPCR and immunohistochemistry staining. The morphologic changes in renal tissues of C/EBPα-/- mice and wide type (WT) mice aged 6-8 weeks were observed using H-E staining and Masson staining. Results: The genotype of the second generation offspring of two types of transgenic mice accorded with the Mendelian laws and C/EBPα-/- mice were generated. C/EBPα-/- mice had lower mRNA and protein expressions of C/EBPα in renal tissues and no significant renal morphologic changes compared with WT mice. Conclusion: Cre-loxp system can successfully knock out the C/EBPα gene in mouse renal tubular epithelial cells. C/EBPα-/- mice have no significant renal morphologic changes in baseline condition.

AB - Objective: To generate renal tubular epithelial cell C/EBPα conditional knockout mice, perform phenotypic identification, and lay a foundation for further study on the role of C/EBPα in renal interstitial fibrosis. Methods: C/EBPαflox/flox and pepck-Cre+ transgenic mice were bred and hybridized. The first generation offspring were mated to reproduce the second generation. Mice with C/EBPαflox/flox. pepck-Cre+ genotype (C/EBPα-/- mice) were identified using PCR. The mRNA and protein expressions of C/EBPα in renal tissues were measured using RT-qPCR and immunohistochemistry staining. The morphologic changes in renal tissues of C/EBPα-/- mice and wide type (WT) mice aged 6-8 weeks were observed using H-E staining and Masson staining. Results: The genotype of the second generation offspring of two types of transgenic mice accorded with the Mendelian laws and C/EBPα-/- mice were generated. C/EBPα-/- mice had lower mRNA and protein expressions of C/EBPα in renal tissues and no significant renal morphologic changes compared with WT mice. Conclusion: Cre-loxp system can successfully knock out the C/EBPα gene in mouse renal tubular epithelial cells. C/EBPα-/- mice have no significant renal morphologic changes in baseline condition.

KW - C/EBPα

KW - Conditional knockout

KW - Cre-loxp system

KW - Renal tubular epithelial cells

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JF - Journal of Shanghai Jiaotong University (Medical Science)

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Generation and identification of renal tubular epithelial cell C/EBPα conditional knockout mice

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Keywords

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