Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that selectively labels developing thymus primordium

Wataru Kimura, Mohammad Tofael Kabir Sharkar, Nishat Sultana, Mohammod Johirul Islam, Tadayoshi Uezato, Naoyuki Miura

Research output: Contribution to journalArticlepeer-review

Abstract

Thymus development is a complicated process that includes highly dynamic morphological changes and reciprocal tissue interactions between endoderm-derived epithelial cells of the anterior foregut and neural crest-derived mesenchymal cells. We generated and characterized a Tbx1-AmCyan1 reporter transgenic mouse to visualize thymus precursor cells during early embryonic development. In transgenic embryos, AmCyan1 fluorescence was specifically detected in the endoderm of the developing 3rd and 4th pharyngeal pouches and later in thymus epithelium until E14. 5. Cells expressing AmCyan1 that were isolated based on AmCyan1 fluorescence expressed endodermal, thymic, and parathyroid markers, but they did not express neural crest or endothelial markers; these findings indicated that this transgenic mouse strain could be used to collect thymic or parathyroid precursor cells or both. We also showed that in nude mice, which exhibit defects in thymus development, the thymus precursors were clearly labeled with AmCyan1. In summary, these AmCyan1-fluorescent transgenic mice are useful for investigating early thymus development.

Original languageEnglish
Pages (from-to)659-666
Number of pages8
JournalTransgenic Research
Volume22
Issue number3
DOIs
StatePublished - Jun 2013
Externally publishedYes

Keywords

  • Anti-AmCyan1 antibody
  • Cell isolation
  • Endoderm
  • Parathyroid
  • Pharyngeal pouch
  • Thymus

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