Gene transfer in cardiomyocytes derived from ES and iPS cells

Francesca Stillitano, Ioannis Karakikes, Roger J. Hajjar

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies. The ability to genetically manipulate hiPSC-CMs not only is essential for identifying the structural and/or functional role of a protein but can also provide valuable information regarding therapeutic applications. In this chapter, we describe protocols for culture, maintenance, and cardiac differentiation of hiPSCs. Then, we provide a basic procedure to transduce hiPSC-CMs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cardiac differentiation
  • Gene therapy
  • HPSC-derived cardiomyocytes
  • Human pluripotent stem cells (hPSCs)
  • Transduction


Dive into the research topics of 'Gene transfer in cardiomyocytes derived from ES and iPS cells'. Together they form a unique fingerprint.

Cite this