Gene-protein correlation in single cells

Jaclyn J. Renfrow; Adrienne C. Scheck; Neil S. Dhawan; Paul J. Lukac; Hannes Vogel; James P. Chandler; Jeffrey J. Raizer; Griffith R. Harsh; Arnab Chakravarti; Markus Bredel

doi:10.1093/neuonc/nor071

Gene-protein correlation in single cells

Jaclyn J. Renfrow, Adrienne C. Scheck, Neil S. Dhawan, Paul J. Lukac, Hannes Vogel, James P. Chandler, Jeffrey J. Raizer, Griffith R. Harsh, Arnab Chakravarti, Markus Bredel

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.

Original language	English
Pages (from-to)	880-885
Number of pages	6
Journal	Neuro-Oncology
Volume	13
Issue number	8
DOIs	https://doi.org/10.1093/neuonc/nor071
State	Published - Aug 2011
Externally published	Yes

Keywords

Epidermal growth factor receptor
Fluorescence in situ gene protein assay
Fluorescence in situ hybridization
Gene amplification
Glioblastoma
Proximity ligation assay

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10.1093/neuonc/nor071

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title = "Gene-protein correlation in single cells",

abstract = "We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.",

keywords = "Epidermal growth factor receptor, Fluorescence in situ gene protein assay, Fluorescence in situ hybridization, Gene amplification, Glioblastoma, Proximity ligation assay",

author = "Renfrow, {Jaclyn J.} and Scheck, {Adrienne C.} and Dhawan, {Neil S.} and Lukac, {Paul J.} and Hannes Vogel and Chandler, {James P.} and Raizer, {Jeffrey J.} and Harsh, {Griffith R.} and Arnab Chakravarti and Markus Bredel",

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month = aug,

doi = "10.1093/neuonc/nor071",

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T1 - Gene-protein correlation in single cells

AU - Renfrow, Jaclyn J.

AU - Scheck, Adrienne C.

AU - Dhawan, Neil S.

AU - Lukac, Paul J.

AU - Vogel, Hannes

AU - Chandler, James P.

AU - Raizer, Jeffrey J.

AU - Harsh, Griffith R.

AU - Chakravarti, Arnab

AU - Bredel, Markus

PY - 2011/8

Y1 - 2011/8

N2 - We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.

AB - We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.

KW - Epidermal growth factor receptor

KW - Fluorescence in situ gene protein assay

KW - Fluorescence in situ hybridization

KW - Gene amplification

KW - Glioblastoma

KW - Proximity ligation assay

UR - http://www.scopus.com/inward/record.url?scp=80052705932&partnerID=8YFLogxK

U2 - 10.1093/neuonc/nor071

DO - 10.1093/neuonc/nor071

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SN - 1522-8517

VL - 13

SP - 880

EP - 885

JO - Neuro-Oncology

JF - Neuro-Oncology

IS - 8

ER -

Gene-protein correlation in single cells

Abstract

Keywords

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