GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst

Nadine Schrode, Néstor Saiz, Stefano Di Talia, Anna Katerina Hadjantonakis

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

Cells of the inner cell mass (ICM) of the mouse blastocyst differentiate into the pluripotent epiblast or the primitive endoderm (PrE), marked by the transcription factors NANOG and GATA6, respectively. To investigate the mechanistic regulation of this process, we applied an unbiased, quantitative, single-cell-resolution image analysis pipeline to analyze embryos lacking or exhibiting reduced levels of GATA6. We find that Gata6 mutants exhibit a complete absence of PrE and demonstrate that GATA6 levels regulate the timing and speed of lineage commitment within the ICM. Furthermore, we show that GATA6 is necessary for PrE specification by FGF signaling and propose a model where interactions between NANOG, GATA6, and the FGF/ERK pathway determine ICM cell fate. This study provides a framework for quantitative analyses of mammalian embryos and establishes GATA6 as a nodal point in the gene regulatory network driving ICM lineage specification.

Original languageEnglish
Pages (from-to)454-467
Number of pages14
JournalDevelopmental Cell
Volume29
Issue number4
DOIs
StatePublished - 27 May 2014
Externally publishedYes

Fingerprint

Dive into the research topics of 'GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst'. Together they form a unique fingerprint.

Cite this