Gata4 is necessary for normal pulmonary lobar development

Kate G. Ackerman, Jianlong Wang, Liqing Luo, Yuko Fujiwara, Stuart H. Orkin, David R. Beier

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Mutations of Fog2 in mice result in a phenotype that includes pulmonary lobar defects. To determine whether formation of the accessory lobe bronchus is mediated by a Gata family cofactor, we evaluated embryonic lungs from mice carrying missense mutations that cause loss of FOG-GATA protein interaction. Lungs from embryos carrying a missense mutation in Gata6 were structurally normal, while lungs from embryos carrying mutations of either Gata4 or of both Gata4 and Gata6 had a structural phenotype that matched the Fog2 mutant phenotype. Expression analysis showed that Gata4 and Fog2 are expressed in the ventral and medial pulmonary mesenchyme during secondary budding. Although Gata4 has not previously been suspected as playing a role in lung development, we have found that a Fog2-Gata4 interaction is critical for the development of normal pulmonary lobar structure, and this phenotype is not influenced by the additional loss of Gata6 interaction. Fog2 and Gata4 in the early pulmonary mesenchyme participate in patterning the secondary bronchus of the accessory lobe.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume36
Issue number4
DOIs
StatePublished - Apr 2007

Keywords

  • Accessory lobe
  • Branching
  • Fog
  • Gata
  • Lung

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