TY - JOUR
T1 - GATA-1 binding sites mapped in the β-globin locus by using mammalian chlp-chip analysis
AU - Horak, Christine E.
AU - Mahajan, Milind C.
AU - Luscombe, Nicholas M.
AU - Gerstein, Mark
AU - Weissman, Sherman M.
AU - Snyder, Michael
PY - 2002/3/5
Y1 - 2002/3/5
N2 - The expression of the β-like globin genes is intricately regulated by a series of both general and tissue-restricted transcription factors. The hemapoietic lineage-specific transcription factor GATA-1 is important for erythroid differentiation and has been implicated in regulating the expression of the erythroid-specific genes including the genes of the β-globin locus. In the human erythroleukemic K562 cell line, only one DNA region has been identified previously as a putative site of GATA-1 interaction by in vivo footprinting studies. We mapped GATA-1 binding throughout the β-globin locus by using chlp-chip analysis of K562 cells. We found that GATA-1 binds in a region encompassing the HS2 core element, as was previously identified, and an additional region of GATA-1 binding upstream of the γG gene. This approach will be of general utility for mapping transcription factor binding sites within the β-globin locus and throughout the genome.
AB - The expression of the β-like globin genes is intricately regulated by a series of both general and tissue-restricted transcription factors. The hemapoietic lineage-specific transcription factor GATA-1 is important for erythroid differentiation and has been implicated in regulating the expression of the erythroid-specific genes including the genes of the β-globin locus. In the human erythroleukemic K562 cell line, only one DNA region has been identified previously as a putative site of GATA-1 interaction by in vivo footprinting studies. We mapped GATA-1 binding throughout the β-globin locus by using chlp-chip analysis of K562 cells. We found that GATA-1 binds in a region encompassing the HS2 core element, as was previously identified, and an additional region of GATA-1 binding upstream of the γG gene. This approach will be of general utility for mapping transcription factor binding sites within the β-globin locus and throughout the genome.
UR - http://www.scopus.com/inward/record.url?scp=0037022675&partnerID=8YFLogxK
U2 - 10.1073/pnas.052706999
DO - 10.1073/pnas.052706999
M3 - Article
C2 - 11867748
AN - SCOPUS:0037022675
SN - 0027-8424
VL - 99
SP - 2924
EP - 2929
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 5
ER -