GATA-1 binding sites mapped in the β-globin locus by using mammalian chlp-chip analysis

Christine E. Horak, Milind C. Mahajan, Nicholas M. Luscombe, Mark Gerstein, Sherman M. Weissman, Michael Snyder

Research output: Contribution to journalArticlepeer-review

136 Scopus citations


The expression of the β-like globin genes is intricately regulated by a series of both general and tissue-restricted transcription factors. The hemapoietic lineage-specific transcription factor GATA-1 is important for erythroid differentiation and has been implicated in regulating the expression of the erythroid-specific genes including the genes of the β-globin locus. In the human erythroleukemic K562 cell line, only one DNA region has been identified previously as a putative site of GATA-1 interaction by in vivo footprinting studies. We mapped GATA-1 binding throughout the β-globin locus by using chlp-chip analysis of K562 cells. We found that GATA-1 binds in a region encompassing the HS2 core element, as was previously identified, and an additional region of GATA-1 binding upstream of the γG gene. This approach will be of general utility for mapping transcription factor binding sites within the β-globin locus and throughout the genome.

Original languageEnglish
Pages (from-to)2924-2929
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number5
StatePublished - 5 Mar 2002
Externally publishedYes


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