Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells of Mastomys natalensis

Masakyo Asahara; Yoshikazu Kinoshita; Hirohisa Nakata; Yumi Matsushima; Yoko Naribayashi; Akira Nakamura; Toshimitsu Matsui; Kazuo Chihara; Jun Yamamoto; Atsushi Ichikawa; Tsutomu Chiba

doi:10.1007/BF02090363

Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells of Mastomys natalensis

Masakyo Asahara, Yoshikazu Kinoshita, Hirohisa Nakata, Yumi Matsushima, Yoko Naribayashi, Akira Nakamura, Toshimitsu Matsui, Kazuo Chihara, Jun Yamamoto, Atsushi Ichikawa, Tsutomu Chiba

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Although gastric enterochromaffin-like (ECL) carcinoid tumors are known to develop in patients with long-standing hypergastrinemia, the expression of the gastrin receptor gene in ECL cells has not yet been demonstrated. Therefore, this study was designed to examine gastrin receptor gene expression in ECL cells. Mastomys gastric mucosal cells isolated by enzyme dispersion were separated into 10 fractions (F1-10) by centrifugal elutriation. Each fraction was examined histologically to determine whether they contained ECL and/or parietal cells and Northern blot analysis was used to confirm the presence of histidine decarboxylase and H⁺, K⁺-ATPase gene expression. ECL cells were found only in fractions 1 and 2, whereas parietal cells were detected in fractions 6-10. Gastrin receptor gene expression was demonstrated in both parietal cell-rich and ECL cell-rich fractions. In addition, the gastrin receptor cDNA sequences obtained from the two of the fractions (F1 and 8) were identical. These results suggest that gastrin receptor genes are expressed in ECL cells as well as in parietal cells and that these receptors are identical.

Original language	English
Pages (from-to)	2149-2156
Number of pages	8
Journal	Digestive Diseases and Sciences
Volume	39
Issue number	10
DOIs	https://doi.org/10.1007/BF02090363
State	Published - Oct 1994
Externally published	Yes

Keywords

H
K-ATPase
Mastomys natalensis
gastrin receptor
histidine decarboxylase

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10.1007/BF02090363

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title = "Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells of Mastomys natalensis",

abstract = "Although gastric enterochromaffin-like (ECL) carcinoid tumors are known to develop in patients with long-standing hypergastrinemia, the expression of the gastrin receptor gene in ECL cells has not yet been demonstrated. Therefore, this study was designed to examine gastrin receptor gene expression in ECL cells. Mastomys gastric mucosal cells isolated by enzyme dispersion were separated into 10 fractions (F1-10) by centrifugal elutriation. Each fraction was examined histologically to determine whether they contained ECL and/or parietal cells and Northern blot analysis was used to confirm the presence of histidine decarboxylase and H+, K+-ATPase gene expression. ECL cells were found only in fractions 1 and 2, whereas parietal cells were detected in fractions 6-10. Gastrin receptor gene expression was demonstrated in both parietal cell-rich and ECL cell-rich fractions. In addition, the gastrin receptor cDNA sequences obtained from the two of the fractions (F1 and 8) were identical. These results suggest that gastrin receptor genes are expressed in ECL cells as well as in parietal cells and that these receptors are identical.",

keywords = "H, K-ATPase, Mastomys natalensis, gastrin receptor, histidine decarboxylase",

author = "Masakyo Asahara and Yoshikazu Kinoshita and Hirohisa Nakata and Yumi Matsushima and Yoko Naribayashi and Akira Nakamura and Toshimitsu Matsui and Kazuo Chihara and Jun Yamamoto and Atsushi Ichikawa and Tsutomu Chiba",

year = "1994",

month = oct,

doi = "10.1007/BF02090363",

language = "English",

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T1 - Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells of Mastomys natalensis

AU - Asahara, Masakyo

AU - Kinoshita, Yoshikazu

AU - Nakata, Hirohisa

AU - Matsushima, Yumi

AU - Naribayashi, Yoko

AU - Nakamura, Akira

AU - Matsui, Toshimitsu

AU - Chihara, Kazuo

AU - Yamamoto, Jun

AU - Ichikawa, Atsushi

AU - Chiba, Tsutomu

PY - 1994/10

Y1 - 1994/10

N2 - Although gastric enterochromaffin-like (ECL) carcinoid tumors are known to develop in patients with long-standing hypergastrinemia, the expression of the gastrin receptor gene in ECL cells has not yet been demonstrated. Therefore, this study was designed to examine gastrin receptor gene expression in ECL cells. Mastomys gastric mucosal cells isolated by enzyme dispersion were separated into 10 fractions (F1-10) by centrifugal elutriation. Each fraction was examined histologically to determine whether they contained ECL and/or parietal cells and Northern blot analysis was used to confirm the presence of histidine decarboxylase and H+, K+-ATPase gene expression. ECL cells were found only in fractions 1 and 2, whereas parietal cells were detected in fractions 6-10. Gastrin receptor gene expression was demonstrated in both parietal cell-rich and ECL cell-rich fractions. In addition, the gastrin receptor cDNA sequences obtained from the two of the fractions (F1 and 8) were identical. These results suggest that gastrin receptor genes are expressed in ECL cells as well as in parietal cells and that these receptors are identical.

AB - Although gastric enterochromaffin-like (ECL) carcinoid tumors are known to develop in patients with long-standing hypergastrinemia, the expression of the gastrin receptor gene in ECL cells has not yet been demonstrated. Therefore, this study was designed to examine gastrin receptor gene expression in ECL cells. Mastomys gastric mucosal cells isolated by enzyme dispersion were separated into 10 fractions (F1-10) by centrifugal elutriation. Each fraction was examined histologically to determine whether they contained ECL and/or parietal cells and Northern blot analysis was used to confirm the presence of histidine decarboxylase and H+, K+-ATPase gene expression. ECL cells were found only in fractions 1 and 2, whereas parietal cells were detected in fractions 6-10. Gastrin receptor gene expression was demonstrated in both parietal cell-rich and ECL cell-rich fractions. In addition, the gastrin receptor cDNA sequences obtained from the two of the fractions (F1 and 8) were identical. These results suggest that gastrin receptor genes are expressed in ECL cells as well as in parietal cells and that these receptors are identical.

KW - H

KW - K-ATPase

KW - Mastomys natalensis

KW - gastrin receptor

KW - histidine decarboxylase

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DO - 10.1007/BF02090363

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EP - 2156

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 10

ER -

Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells of Mastomys natalensis

Abstract

Keywords

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