Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells of Mastomys natalensis

Masakyo Asahara, Yoshikazu Kinoshita, Hirohisa Nakata, Yumi Matsushima, Yoko Naribayashi, Akira Nakamura, Toshimitsu Matsui, Kazuo Chihara, Jun Yamamoto, Atsushi Ichikawa, Tsutomu Chiba

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52 Scopus citations


Although gastric enterochromaffin-like (ECL) carcinoid tumors are known to develop in patients with long-standing hypergastrinemia, the expression of the gastrin receptor gene in ECL cells has not yet been demonstrated. Therefore, this study was designed to examine gastrin receptor gene expression in ECL cells. Mastomys gastric mucosal cells isolated by enzyme dispersion were separated into 10 fractions (F1-10) by centrifugal elutriation. Each fraction was examined histologically to determine whether they contained ECL and/or parietal cells and Northern blot analysis was used to confirm the presence of histidine decarboxylase and H+, K+-ATPase gene expression. ECL cells were found only in fractions 1 and 2, whereas parietal cells were detected in fractions 6-10. Gastrin receptor gene expression was demonstrated in both parietal cell-rich and ECL cell-rich fractions. In addition, the gastrin receptor cDNA sequences obtained from the two of the fractions (F1 and 8) were identical. These results suggest that gastrin receptor genes are expressed in ECL cells as well as in parietal cells and that these receptors are identical.

Original languageEnglish
Pages (from-to)2149-2156
Number of pages8
JournalDigestive Diseases and Sciences
Issue number10
StatePublished - Oct 1994
Externally publishedYes


  • H
  • K-ATPase
  • Mastomys natalensis
  • gastrin receptor
  • histidine decarboxylase


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