Gametic differentiation in Chlamydomonas Reinhardtii: II. flagellar membranes and the agglutination reaction

Kenneth Bergman, Ursula W. Goodenough, Daniel A. Goodenough, Jack Jawitz, Howard Martin

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

A structural and biochemical study is presented concerning the agglutination of gametic flagella, the initial step in the mating reaction of Chlamydomonas reinhardtii. An alteration in the distribution of the intramembranous particles revealed by freeze-fracturing of flagellar membranes is shown to accompany gametic differentiation in both mating types. The isolation and electrophoretic analysis of flagellar membranes and mastigonemes are reported; no electrophoretic differences can be detected when the membrane or mastigoneme glycoproteins from vegetative and gametic cells are compared, nor when glycoproteins from the two mating types are compared, and no novel polypeptides are present in gametic preparations. The membrane vesicles, after they are freed of mastigonemes by sedimentation through a discontinuous sucrose gradient, are extremely active as an isoagglutinin, indicating a direct involvement of the membrane in the mating reaction.

Original languageEnglish
Pages (from-to)606-622
Number of pages17
JournalJournal of Cell Biology
Volume67
Issue number3
DOIs
StatePublished - 1 Dec 1975
Externally publishedYes

Fingerprint

Dive into the research topics of 'Gametic differentiation in Chlamydomonas Reinhardtii: II. flagellar membranes and the agglutination reaction'. Together they form a unique fingerprint.

Cite this