TY - JOUR
T1 - Further studies on the differences in serum dependence in EBV negative lymphoma lines and their in vitro EBV converted, virus-genome carrying sublines
AU - Steinitz, Michael
AU - Klein, George
N1 - Funding Information:
Accepted 27 April 1977. *This work was undertaken during the tenure of a fellowship awarded by the European Molecular Biology Organization ~o Michael Steinitz. It was supported by Contract No. NOI CP 33316 within the Virus Cancer Program of the National Cancer Institute.
PY - 1977/11
Y1 - 1977/11
N2 - Independently EBV-converted, viral genome carrying sublines of the originally EBV negative Ramos and BJAB lymphoma lines showed decreased serum dependence, in comparison with the progenitor lines. The virus negative lines could not grow in 10% dialysed FCS, unless reconstituted with the dialysate. The converted lines grew well on dialysed serum. Seeding of the EBV negative lines with larger inocula enabled them to "take off" on dialysed serum as well. The EBV converted lines were able to form colonies in soft agar, whereas the original negative lines failed to do so.
AB - Independently EBV-converted, viral genome carrying sublines of the originally EBV negative Ramos and BJAB lymphoma lines showed decreased serum dependence, in comparison with the progenitor lines. The virus negative lines could not grow in 10% dialysed FCS, unless reconstituted with the dialysate. The converted lines grew well on dialysed serum. Seeding of the EBV negative lines with larger inocula enabled them to "take off" on dialysed serum as well. The EBV converted lines were able to form colonies in soft agar, whereas the original negative lines failed to do so.
UR - http://www.scopus.com/inward/record.url?scp=0017674492&partnerID=8YFLogxK
U2 - 10.1016/0014-2964(77)90035-4
DO - 10.1016/0014-2964(77)90035-4
M3 - Article
C2 - 201467
AN - SCOPUS:0017674492
SN - 0014-2964
VL - 13
SP - 1269
EP - 1275
JO - European Journal of Cancer (1965)
JF - European Journal of Cancer (1965)
IS - 11
ER -