Further characterization of the putative glycolipid receptor for mif: Role of fucose associated with an acidic glycolipid

Terry J. Higgins, David Y. Liu, Heinz G. Remold, John R. David

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Water soluble glycolipids were extracted from guinea pig macrophages. These glycolipids, when incubated with macrophages, augment the cells' response to migration inhibitory factor. The glycolipids were fractionated by diethylaminoethyl-Sephadex ion exchange chromatography into neutral and acidic fractions. Only the acidic glycolipid fraction was able to enhance the responsiveness of macrophages to migration inhibitory factor. Additional studies indicate that the enhancing activity of these glycolipid preparations can be abrogated by the removal of terminal fucose residues with α-L-fucosidase. The possibility that fucose functions as an essential component of a macrophage glycolipid receptor for migration inhibitory factor is discussed.

Original languageEnglish
Pages (from-to)1259-1265
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume93
Issue number4
DOIs
StatePublished - 29 Apr 1980
Externally publishedYes

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