Functional role of the ultraviolet light responsive element (URE; TGACAACA) in the transcription and replication of polyoma DNA

Susan E. Rutberg, Yang M. Yang, Zeev Ronai

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We have previously Identified a novel 8 bp sequence (UV-responsive element, URE: TGACAACA) present in the regulatory region of polyoma DNA that Interacts with protein factors induced in rat fibroblast cells by exposure to UV light. In the present study, wedemonstrate through competitive binding assays that this sequence Is distinct from the partially homologous AP1 and CRE target sequences. The proteins that bind to the URE appear to have transcriptional activity in UVexposed rat flbroblasts. In addition, the URE appearsto play a role in promoting the replication of polyoma DNA as determined through two different experimental approaches. Together, these findings suggest that the URE is a novel DNA binding element that interacts with proteins involved in the transcription and replication of polyoma sequences.

Original languageEnglish
Pages (from-to)4305-4310
Number of pages6
JournalNucleic Acids Research
Volume20
Issue number16
DOIs
StatePublished - 25 Aug 1992
Externally publishedYes

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