@article{d3c41477c0784b6e9c11b65eacc44bb8,
title = "Functional effects of the mouse weaver mutation on G protein-gated inwardly rectifying K+ channels",
abstract = "The weaver mutation corresponds to a substitution of glycine to serine in the H5 region of a G protein-gated inwardly rectifying K+ channel gene (GIRK2). By studying mutant GIRK2 weaver homomultimeric channels and heteromultimeric channels comprised of GIRK2 weaver and GIRK1 in Xenopus oocytes, we found that GIRK2 weaver homomultimeric channels lose their selectivity for K+ ions, giving rise to inappropriate receptor-activated and basally active Na+ currents, whereas heteromultimers of GIRK2 weaver and GIRK1 appeared to have reduced current. Immunohistochemical localization indicates that GIRK2 and GIRK1 proteins are expressed in the cerebellar neurons of mice at postnatal day 4, at a time when these neurons normally undergo differentiation. Thus, the aberrant behavior of mutant GIRK2 weaver channels could affect the development of weaver mice in at least two distinct ways.",
author = "Slesinger, {Paul A.} and Nila Patil and Liao, {Y. Joyce} and Jan, {Yuh Nung} and Jan, {Lily Y.} and Cox, {David R.}",
note = "Funding Information: Correspondence should be addressed to P. A. S. We thank Drs. A. Connolly for providing β 1 and γ 2 cDNA clones, H. A. Lester for providing m2 receptor, M. Lazdunski for providing mGIRK2 in high expression vector, D. E. Clapham for providing a preprint, M. J. Bradbury for critically reading this manuscript, and E. Wolff for her technical assistance. This work was supported by a National Institute of Mental Health grant to the Silvio Conte Neuroscience Center at the University of California, San Francisco (UCSF) (Y. N. J. and L. Y. J.), by the UCSF Neuroscience Graduate Program (Y. J. L.), and by National Institutes of Health grant HD24610 (D. R. C.). Y. N. J. and L. Y. J. are Investigators and P. A. S. is an Associate of the Howard Hughes Medical Institute. ",
year = "1996",
month = feb,
doi = "10.1016/S0896-6273(00)80050-1",
language = "English",
volume = "16",
pages = "321--331",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "2",
}