TY - JOUR
T1 - FSH-receptor isoforms and FSH-dependent gene transcription in human monocytes and osteoclasts
AU - Robinson, Lisa J.
AU - Tourkova, Irina
AU - Wang, Yujuan
AU - Sharrow, Allison C.
AU - Landau, Michael S.
AU - Yaroslavskiy, Beatrice B.
AU - Sun, Li
AU - Zaidi, Mone
AU - Blair, Harry C.
N1 - Funding Information:
Supported by National Institutes of Health Grants AR053976 , AR055208 , AR053566 and by the Department of Veteran’s Affairs (USA). M.Z. and L.S. also acknowledge National Institutes of Health Grants AG23176 , DK70526 , and DK70526 . MZ is a named inventor of a pending patent application related to osteoclastic bone resorption filed by the Mount Sinai School of Medicine (MSSM). In the event the pending or issued patent is licensed, he would be entitled to a share of any proceeds MSSM receives from the licensee.
PY - 2010/3/26
Y1 - 2010/3/26
N2 - Cells of the monocyte series respond to follicle stimulating hormone (FSH) by poorly characterized mechanisms. We studied FSH-receptors (FSH-R) and FSH response in nontransformed human monocytes and in osteoclasts differentiated from these cells. Western blot and PCR confirmed FSH-R expression on monocytes or osteoclasts, although at low levels relative to ovarian controls. Monocyte and osteoclast FSH-Rs differed from FSH-R from ovarian cells, reflecting variable splicing in exons 8-10. Monocytes produced no cAMP, the major signal in ovarian cells, in response to FSH. However, monocytes and osteoclasts transcribed TNFα in response to the FSH. No relation of expression of osteoclast FSH-R to the sex of cell donors or to exposure to sex hormones was apparent. Controls for FSH purity and endotoxin contamination were negative. Unamplified cRNA screening in adherent CD14 cells after 2 h in 25 ng/ml FSH showed increased transcription of RANKL signalling proteins. Transcription of key proteins that stimulate bone turnover, TNFα and TSG-6, increased 2- to 3-fold after FSH treatment. Smaller but significant changes occurred in transcripts of selected signalling, adhesion, and cytoskeletal proteins. We conclude that monocyte and osteoclast FSH response diverges from that of ovarian cells, reflecting, at least in part, varying FSH-R isoforms.
AB - Cells of the monocyte series respond to follicle stimulating hormone (FSH) by poorly characterized mechanisms. We studied FSH-receptors (FSH-R) and FSH response in nontransformed human monocytes and in osteoclasts differentiated from these cells. Western blot and PCR confirmed FSH-R expression on monocytes or osteoclasts, although at low levels relative to ovarian controls. Monocyte and osteoclast FSH-Rs differed from FSH-R from ovarian cells, reflecting variable splicing in exons 8-10. Monocytes produced no cAMP, the major signal in ovarian cells, in response to FSH. However, monocytes and osteoclasts transcribed TNFα in response to the FSH. No relation of expression of osteoclast FSH-R to the sex of cell donors or to exposure to sex hormones was apparent. Controls for FSH purity and endotoxin contamination were negative. Unamplified cRNA screening in adherent CD14 cells after 2 h in 25 ng/ml FSH showed increased transcription of RANKL signalling proteins. Transcription of key proteins that stimulate bone turnover, TNFα and TSG-6, increased 2- to 3-fold after FSH treatment. Smaller but significant changes occurred in transcripts of selected signalling, adhesion, and cytoskeletal proteins. We conclude that monocyte and osteoclast FSH response diverges from that of ovarian cells, reflecting, at least in part, varying FSH-R isoforms.
KW - Cofilin
KW - Fibronectin
KW - Inositol-(1,4,5)-trisphosphate receptor 1
KW - PLAUR urokinase receptor
KW - Tumor necrosis factor-stimulated gene sequence-6
UR - https://www.scopus.com/pages/publications/77949873305
U2 - 10.1016/j.bbrc.2010.02.112
DO - 10.1016/j.bbrc.2010.02.112
M3 - Article
C2 - 20171950
AN - SCOPUS:77949873305
SN - 0006-291X
VL - 394
SP - 12
EP - 17
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -