Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β-catenin signaling pathway in ovariectomized rats

Haixia Liu, Yubo Guo, Ruyuan Zhu, Lili Wang, Beibei Chen, Yimiao Tian, Rui Li, Rufeng Ma, Qiangqiang Jia, Hao Zhang, Bingke Xia, Yu Li, Xinxiang Wang, Xiaofeng Zhu, Ronghua Zhang, Dieter Brӧmme, Sihua Gao, Dongwei Zhang, Xiaohua Pei

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Fructus Ligustri Lucidi (FLL) has been preclinically and clinically used to treat musculoskeletal diseases. However, whether and how FLL affect the canonical Wnt/β-catenin signaling in the management of osteoporosis remains largely unknown. To this end, ovariectomized (OVX) rats and primary osteoblasts were administrated with FLL aqueous extract and medicated serum, respectively. Supplement of FLL to OVX rats maintains bone quality by attenuating the reduction in bone mineral density, strength and microstructure. The maintenance may be associated with upregulating the expression of insulin-like growth factor-1, osteoprotegerin, phospho (p)-low-density lipoprotein receptor-related protein 6, p-glycogen synthase kinase 3 beta (GSK3β), β-catenin, Runx2 and c-Myc, and downregulating the expressions of sclerostin (SOST), dickkopf-related protein 1 (DKK1), GSK3β and p-β-catenin in rat femurs and tibias. In addition, the medicated serum promotes osteoblastic bone formation through activation of Wnt/β-catenin signaling via inhibition of DKK1 and SOST overexpression. Salidroside may be one of the active ingredients in FLL that are beneficial for bone homeostasis. In summary, our results suggest that FLL may preserve bone quality through induction of canonical Wnt/β-catenin signaling via inhibition of DKK1 and SOST overexpression. And FLL may offer a new source of the DKK1 or SOST inhibitors in protection against osteoporosis.

Original languageEnglish
Pages (from-to)424-441
Number of pages18
JournalPhytotherapy Research
Volume35
Issue number1
DOIs
StatePublished - Jan 2021
Externally publishedYes

Keywords

  • Fructus Ligustri Lucidi
  • Wnt/β-catenin
  • bone quality
  • medicated serum
  • ovariectomized rats
  • primary osteoblasts

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