FRET acceptor suppressed single-particle photobleaching in semiconductor polymer dots

Zezhou Sun, Siyang Liu, Zhihe Liu, Weiping Qin, Danni Chen, Gaixia Xu, Changfeng Wu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Brightness and photostability are key parameters for fluorescent probes in optical imaging. This Letter describes Förster resonance energy transfer (FRET) as a useful strategy to enhance the photostability of fluorescent nanoparticles. Small molecules as FRET acceptors were doped into semiconductor polymer dots (Pdots), yielding apparent suppression of their rapid photobleaching in single-particle imaging. For 20 nmdiameter particles, the photobleaching percentage decreased from 71.8% to 47.2% after dye doping, while the singleparticle brightness remained unchanged. The photostability of large Pdots was also enhanced by FRET at the expense of a moderate decrease in per-particle brightness as compared to the pure Pdots. This study indicates that FRET is a facile, yet effective, approach to mediate the brightness and photostability of fluorescent nanoparticles. Considering the combined factors of brightness and photostability, the dye-doped Pdots of ?20 nm diameter are the most suitable for long-term imaging and tracking applications.

Original languageEnglish
Pages (from-to)2370-2373
Number of pages4
JournalOptics Letters
Issue number10
StatePublished - 15 May 2016
Externally publishedYes


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