Fragment-based de novo design of a cystathionine γ-lyase selective inhibitor blocking hydrogen sulfide production

Angela Corvino, Beatrice Severino, Ferdinando Fiorino, Francesco Frecentese, Elisa Magli, Elisa Perissutti, Vincenzo Santagada, Mariarosaria Bucci, Giuseppe Cirino, Geoff Kelly, Luigi Servillo, Grzegorz Popowicz, Annalisa Pastore, Giuseppe Caliendo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Hydrogen sulfide is an essential catabolite that intervenes in the pathophysiology of several diseases from hypertension to stroke, diabetes and pancreatitis. It is endogenously synthesized mainly by two pyridoxal-5′-phosphate-dependent enzymes involved in L-cysteine metabolism: cystathionine-ß-synthase (CBS) and cystathionine-γ-lyase (CSE). Research in this field is currently impaired by the lack of pharmacological tools such as selective enzymatic inhibitors that could target specifically only one of these pathways. We used a novel approach based on a hybrid method that includes drug design, synthetic biology, metabolomics and pharmacological assays to rationally design a new inhibitor selective for the CSE enzyme. The identification of this compound opens new frontiers towards a better understanding of the role of CSE over CBS in the pathophysiology of diseases where a role for the H 2 S pathway has been proposed and the development of new lead compounds that could target the CSE enzyme.

Original languageEnglish
Article number34398
JournalScientific Reports
Volume6
DOIs
StatePublished - 6 Oct 2016
Externally publishedYes

Fingerprint

Dive into the research topics of 'Fragment-based de novo design of a cystathionine γ-lyase selective inhibitor blocking hydrogen sulfide production'. Together they form a unique fingerprint.

Cite this