TY - JOUR
T1 - Foxa2 identifies a cardiac progenitor population with ventricular differentiation potential
AU - Bardot, Evan
AU - Calderon, Damelys
AU - Santoriello, Francis
AU - Han, Songyan
AU - Cheung, Kakit
AU - Jadhav, Bharati
AU - Burtscher, Ingo
AU - Artap, Stanley
AU - Jain, Rajan
AU - Epstein, Jonathan
AU - Lickert, Heiko
AU - Gouon-Evans, Valerie
AU - Sharp, Andrew J.
AU - Dubois, Nicole C.
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/2/14
Y1 - 2017/2/14
N2 - The recent identification of progenitor populations that contribute to the developing heart in a distinct spatial and temporal manner has fundamentally improved our understanding of cardiac development. However, the mechanisms that direct atrial versus ventricular specification remain largely unknown. Here we report the identification of a progenitor population that gives rise primarily to cardiovascular cells of the ventricles and only to few atrial cells (<5%) of the differentiated heart. These progenitors are specified during gastrulation, when they transiently express Foxa2, a gene not previously implicated in cardiac development. Importantly, Foxa2+ cells contribute to previously identified progenitor populations in a defined pattern and ratio. Lastly, we describe an analogous Foxa2+ population during differentiation of embryonic stem cells. Together, these findings provide insight into the developmental origin of ventricular and atrial cells, and may lead to the establishment of new strategies for generating chamber-specific cell types from pluripotent stem cells.
AB - The recent identification of progenitor populations that contribute to the developing heart in a distinct spatial and temporal manner has fundamentally improved our understanding of cardiac development. However, the mechanisms that direct atrial versus ventricular specification remain largely unknown. Here we report the identification of a progenitor population that gives rise primarily to cardiovascular cells of the ventricles and only to few atrial cells (<5%) of the differentiated heart. These progenitors are specified during gastrulation, when they transiently express Foxa2, a gene not previously implicated in cardiac development. Importantly, Foxa2+ cells contribute to previously identified progenitor populations in a defined pattern and ratio. Lastly, we describe an analogous Foxa2+ population during differentiation of embryonic stem cells. Together, these findings provide insight into the developmental origin of ventricular and atrial cells, and may lead to the establishment of new strategies for generating chamber-specific cell types from pluripotent stem cells.
UR - http://www.scopus.com/inward/record.url?scp=85012936376&partnerID=8YFLogxK
U2 - 10.1038/ncomms14428
DO - 10.1038/ncomms14428
M3 - Article
C2 - 28195173
AN - SCOPUS:85012936376
SN - 2041-1723
VL - 8
JO - Nature Communications
JF - Nature Communications
M1 - 14428
ER -