Fourier transform infrared spectroscopy based spectral biomarkers of metastasized breast cancer progression

  • Sumanta Kar
  • , Dinesh R. Katti
  • , Kalpana S. Katti

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Breast cancer is a global health issue and the second leading cause of cancer death in women. Breast cancer tends to migrate to bone and causes bone metastases which is ultimately the cause of death. Here, we report the use of FTIR to identify spectral biomarkers of cancer progression on 3D in vitro model of breast cancer bone metastasis. Our results indicate that the following spectral biomarkers can monitor cancer progression, for example, lipids (CH2 asymmetric/CH2 symmetric stretch), Amide I/Amide II, and RNA/DNA. Principal component analysis also confirmed the involvement of protein, lipids and nucleic acids in cancer progression on sequential culture. The collective observations from this study suggest successful application of FTIR as a non-invasive and accurate method to identify biochemical changes in cancer cells during the progression of breast cancer bone metastasis.

Original languageEnglish
Pages (from-to)85-96
Number of pages12
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume208
DOIs
StatePublished - 5 Feb 2019
Externally publishedYes

Keywords

  • 3D in vitro model
  • Bone metastases
  • Breast cancer
  • FTIR spectroscopy
  • Principal component analysis

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