Fos-expressing neuronal ensemble in rat ventromedial prefrontal cortex encodes cocaine seeking but not food seeking in rats

Louisa Kane, Marco Venniro, Richard Quintana-Feliciano, Rajtarun Madangopal, F. Javier Rubio, Jennifer M. Bossert, Daniele Caprioli, Yavin Shaham, Bruce T. Hope, Brandon L. Warren

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Neuronal ensembles in ventromedial prefrontal cortex (vmPFC) play a role in both cocaine and palatable food seeking. However, it is unknown whether similar or different vmPFC neuronal ensembles mediate food and cocaine seeking. Here, we used the Daun02 inactivation procedure to assess whether the neuronal ensembles mediating food and cocaine seeking can be functionally distinguished. We trained male and female Fos-LacZ rats to self-administer palatable food pellets and cocaine on alternating days for 18 days. We then exposed the rats to a brief nonreinforced food- or cocaine-seeking test to induce Fos and β-gal in neuronal ensembles associated with food or cocaine seeking, respectively and infused Daun02 into vmPFC to ablate the β-gal-expressing ensembles. Two days later, we tested the rats for food or cocaine seeking under extinction conditions. Although inactivation of the food-seeking ensemble did not influence food or cocaine seeking, inactivation of the cocaine-seeking ensemble reduced cocaine seeking but not food seeking. Results indicate that the neuronal ensemble activated by cocaine seeking in vmPFC is functionally separate from the ensemble activated by food seeking.

Original languageEnglish
Article numbere12943
JournalAddiction Biology
Volume26
Issue number3
DOIs
StatePublished - May 2021
Externally publishedYes

Keywords

  • Daun02 inactivation
  • addiction
  • operant conditioning
  • vmPFC

Fingerprint

Dive into the research topics of 'Fos-expressing neuronal ensemble in rat ventromedial prefrontal cortex encodes cocaine seeking but not food seeking in rats'. Together they form a unique fingerprint.

Cite this