TY - JOUR
T1 - Food allergy herbal formula 2 protection against peanut anaphylactic reaction is via inhibition of mast cells and basophils
AU - Song, Ying
AU - Qu, Chunfeng
AU - Srivastava, Kamal
AU - Yang, Nan
AU - Busse, Paula
AU - Zhao, Wei
AU - Li, Xiu Min
N1 - Funding Information:
Supported by the Dugan Family Foundation, the Food Allergy Initiative, and National Institutes of Health grant no. 1R01AT001495-01A1 and no. 2 R01 AT001495-05A1 to X.-M.L.
Funding Information:
Disclosure of potential conflict of interest: P. Busse is a consultant for ViroPharma, receives research support from the AAAAI and the NIH , and has provided legal consultation services/expert witness testimony in cases related to hereditary angioedema. W. Zhao receives research support from the NIH . X.-M. Li is a consultant for the Food Allergy Initiative, has received research support from the Food Allergy Initiative and the NIH , and has shares of US patent PCT/US05/08600 on FAHF-2 and Herbal Springs LLC. The rest of the authors have declared that they have no conflict of interest.
PY - 2010/12
Y1 - 2010/12
N2 - Background: Mast cells and basophils are key effector cells of IgE-mediated anaphylactic reactions. The Chinese herbal formula, food allergy herbal formula 2 (FAHF-2), protects against peanut anaphylaxis in mice. However, the mechanisms underlying this effect are not fully elucidated. Objective: To investigate whether FAHF-2 inhibits mast cell/basophil numbers and IgE-mediated activation. Methods: Mice with peanut allergy (PNA mice) were treated with FAHF-2 intragastrically for 7 weeks and challenged intragastrically with peanut 1 day and 4 weeks posttreatment. Peripheral blood basophil numbers and peritoneal mast cell numbers and FcεRI expression were determined. Direct effects of FAHF-2 on the murine mast cell line MC/9, and effects of 4 fractions and 3 compounds isolated from FAHF-2 on rat basophilic leukemia cells (RBL-2H3) and human skin mast cells degranulation and on the IgE-mediated spleen tyrosine kinase signaling pathway, were determined. Results: Although all sham-treated PNA mice developed anaphylaxis, FAHF-2-treated PNA mice were protected against anaphylaxis after peanut challenge at 1 day and 4 weeks posttherapy. Reduction of peripheral blood basophils began after 1 week of treatment and continued for at least 4 weeks posttherapy. The number and FcεRI expression of peritoneal mast cells were also significantly decreased 4 weeks posttherapy. FAHF-2-treated MC/9 cells showed significantly reduced IgE-induced FcεRI expression, FcεRI γ mRNA subunit expression, proliferation, and histamine release on challenge. Fraction 2 from FAHF-2 inhibited RBL-2H3 cell and human mast cell degranulation. Three compounds from fraction 2 - berberine, palmatine, and jatrorrhizine - inhibited RBL-2H3 cell degranulation via suppressing spleen tyrosine kinase phosphorylation. Conclusion: Food allergy herbal formula 2 reduction of basophils and mast cell numbers as well as suppression of IgE-mediated mast cell activation may contribute to FAHF-2's persistent protection against peanut anaphylaxis.
AB - Background: Mast cells and basophils are key effector cells of IgE-mediated anaphylactic reactions. The Chinese herbal formula, food allergy herbal formula 2 (FAHF-2), protects against peanut anaphylaxis in mice. However, the mechanisms underlying this effect are not fully elucidated. Objective: To investigate whether FAHF-2 inhibits mast cell/basophil numbers and IgE-mediated activation. Methods: Mice with peanut allergy (PNA mice) were treated with FAHF-2 intragastrically for 7 weeks and challenged intragastrically with peanut 1 day and 4 weeks posttreatment. Peripheral blood basophil numbers and peritoneal mast cell numbers and FcεRI expression were determined. Direct effects of FAHF-2 on the murine mast cell line MC/9, and effects of 4 fractions and 3 compounds isolated from FAHF-2 on rat basophilic leukemia cells (RBL-2H3) and human skin mast cells degranulation and on the IgE-mediated spleen tyrosine kinase signaling pathway, were determined. Results: Although all sham-treated PNA mice developed anaphylaxis, FAHF-2-treated PNA mice were protected against anaphylaxis after peanut challenge at 1 day and 4 weeks posttherapy. Reduction of peripheral blood basophils began after 1 week of treatment and continued for at least 4 weeks posttherapy. The number and FcεRI expression of peritoneal mast cells were also significantly decreased 4 weeks posttherapy. FAHF-2-treated MC/9 cells showed significantly reduced IgE-induced FcεRI expression, FcεRI γ mRNA subunit expression, proliferation, and histamine release on challenge. Fraction 2 from FAHF-2 inhibited RBL-2H3 cell and human mast cell degranulation. Three compounds from fraction 2 - berberine, palmatine, and jatrorrhizine - inhibited RBL-2H3 cell degranulation via suppressing spleen tyrosine kinase phosphorylation. Conclusion: Food allergy herbal formula 2 reduction of basophils and mast cell numbers as well as suppression of IgE-mediated mast cell activation may contribute to FAHF-2's persistent protection against peanut anaphylaxis.
KW - Chinese herbal medicine
KW - FcεRI
KW - Peanut anaphylaxis
KW - mast cells/basophils
UR - http://www.scopus.com/inward/record.url?scp=78649833836&partnerID=8YFLogxK
U2 - 10.1016/j.jaci.2010.09.013
DO - 10.1016/j.jaci.2010.09.013
M3 - Article
AN - SCOPUS:78649833836
SN - 0091-6749
VL - 126
SP - 1208-1217.e1-e3
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
IS - 6
ER -