Flow cytometric identification of TFH13 cells in mouse and human

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams

Research output: Contribution to journalReview articlepeer-review

10 Scopus citations


Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper TFH13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying TFH13 cells in both mice and humans.

Original languageEnglish
Pages (from-to)470-483
Number of pages14
JournalJournal of Allergy and Clinical Immunology
Issue number2
StatePublished - Feb 2021


  • IL-13
  • IL-4
  • IL-5
  • IgE
  • T follicular helper cells
  • T13 cells
  • allergy
  • intracellular cytokine staining


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