Flow cytometric identification of TFH13 cells in mouse and human

Jennifer S. Chen; Jessica D.S. Grassmann; Uthaman Gowthaman; Sam J. Olyha; Tregony Simoneau; M. Cecilia Berin; Stephanie C. Eisenbarth; Adam Williams

doi:10.1016/j.jaci.2020.04.063

Flow cytometric identification of T_FH13 cells in mouse and human

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams

Research output: Contribution to journal › Review article › peer-review

16 Scopus citations

Abstract

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T_FH13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T_FH13 cells in both mice and humans.

Original language	English
Pages (from-to)	470-483
Number of pages	14
Journal	Journal of Allergy and Clinical Immunology
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/j.jaci.2020.04.063
State	Published - Feb 2021

Keywords

IL-13
IL-4
IL-5
IgE
T follicular helper cells
T13 cells
allergy
intracellular cytokine staining

Access to Document

10.1016/j.jaci.2020.04.063

Cite this

@article{2a39bcc3395d4d1bbde6350102228053,

title = "Flow cytometric identification of TFH13 cells in mouse and human",

abstract = "Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper TFH13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying TFH13 cells in both mice and humans.",

keywords = "IL-13, IL-4, IL-5, IgE, T follicular helper cells, T13 cells, allergy, intracellular cytokine staining",

author = "Chen, {Jennifer S.} and Grassmann, {Jessica D.S.} and Uthaman Gowthaman and Olyha, {Sam J.} and Tregony Simoneau and Berin, {M. Cecilia} and Eisenbarth, {Stephanie C.} and Adam Williams",

note = "Publisher Copyright: {\textcopyright} 2020 American Academy of Allergy, Asthma & Immunology",

year = "2021",

month = feb,

doi = "10.1016/j.jaci.2020.04.063",

language = "English",

volume = "147",

pages = "470--483",

journal = "Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

publisher = "Mosby Inc.",

number = "2",

}

TY - JOUR

T1 - Flow cytometric identification of TFH13 cells in mouse and human

AU - Chen, Jennifer S.

AU - Grassmann, Jessica D.S.

AU - Gowthaman, Uthaman

AU - Olyha, Sam J.

AU - Simoneau, Tregony

AU - Berin, M. Cecilia

AU - Eisenbarth, Stephanie C.

AU - Williams, Adam

PY - 2021/2

Y1 - 2021/2

N2 - Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper TFH13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying TFH13 cells in both mice and humans.

AB - Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper TFH13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying TFH13 cells in both mice and humans.

KW - IL-13

KW - IL-4

KW - IL-5

KW - IgE

KW - T follicular helper cells

KW - T13 cells

KW - allergy

KW - intracellular cytokine staining

UR - http://www.scopus.com/inward/record.url?scp=85088219826&partnerID=8YFLogxK

U2 - 10.1016/j.jaci.2020.04.063

DO - 10.1016/j.jaci.2020.04.063

M3 - Review article

C2 - 32709424

AN - SCOPUS:85088219826

SN - 0091-6749

VL - 147

SP - 470

EP - 483

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

IS - 2

ER -