Abstract
A novel enzyme reactor for phospholipid-dependent reactions was used to study the effects of flow on tissue factor-initiated coagulation. Microcapillaries were coated with a phospholipid bilayer containing tissue factor, a transmembrane protein that is an essential cofactor for a plasma procoagulant enzyme, factor VII. We show that, in contrast to static, closed systems, the steady-state catalytic activity is independent of enzyme concentration and the time to steady state becomes a function of the enzyme concentration.
Original language | English |
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Pages (from-to) | 1404-1406 |
Number of pages | 3 |
Journal | Blood |
Volume | 72 |
Issue number | 4 |
DOIs | |
State | Published - 1988 |
Externally published | Yes |