TY - JOUR
T1 - Fine mapping a major QTL for flag leaf size and yield-related traits in rice
AU - Wang, Peng
AU - Zhou, Guilin
AU - Yu, Huihui
AU - Yu, Sibin
N1 - Funding Information:
This work was supported by grants from the National Natural Science Foundation of China, National Program on Key Basic Research Project, National Special Program for Research of Transgenic Plant of China, and the Gates Foundation.
PY - 2011/12
Y1 - 2011/12
N2 - Leaf size is a major determinant of plant architecture and yield potential in crops. A previous study showed that the genomic region of chromosome 1 contains a major quantitative trait locus (QTL) for flag leaf size in a set of backcross recombinant inbred lines derived from two elite parental lines (Zhenshan 97 and 93-11). In the present study, the QTL (qFL1) was shown to explain a large proportion of the variation in flag leaf size (leaf length, width and area) in derived populations (BC2F3 and BC3F2) in multiple environments. Using a large segregating population, we narrowed the location of qFL1 to a 31 kb region containing four predicted genes. Expression of one of these genes, OsFTL1, differed between leaves in near-isogenic lines carrying alleles of Zhenshan 97 and 93-11. qFL1 had a pleiotropic effect on flag leaf size and yield-related traits. Conditional QTL analysis of the derived population (BC3F2) supports the assertion that qFL1 is the QTL for flag leaf length and exhibits pleiotropy. Pyramiding of qFL1 with two known genes (GS3 and Wx) from 93-11 into Zhenshan 97 enlarged flag leaves, improved grain size and amylose content, and increased yield per plant, but slightly delayed heading date. These results provide a foundation for the functional characterization of the gene underlying the pleiotropic effects of qFL1 and for genetic improvement of the plant architecture and yield potential of rice.
AB - Leaf size is a major determinant of plant architecture and yield potential in crops. A previous study showed that the genomic region of chromosome 1 contains a major quantitative trait locus (QTL) for flag leaf size in a set of backcross recombinant inbred lines derived from two elite parental lines (Zhenshan 97 and 93-11). In the present study, the QTL (qFL1) was shown to explain a large proportion of the variation in flag leaf size (leaf length, width and area) in derived populations (BC2F3 and BC3F2) in multiple environments. Using a large segregating population, we narrowed the location of qFL1 to a 31 kb region containing four predicted genes. Expression of one of these genes, OsFTL1, differed between leaves in near-isogenic lines carrying alleles of Zhenshan 97 and 93-11. qFL1 had a pleiotropic effect on flag leaf size and yield-related traits. Conditional QTL analysis of the derived population (BC3F2) supports the assertion that qFL1 is the QTL for flag leaf length and exhibits pleiotropy. Pyramiding of qFL1 with two known genes (GS3 and Wx) from 93-11 into Zhenshan 97 enlarged flag leaves, improved grain size and amylose content, and increased yield per plant, but slightly delayed heading date. These results provide a foundation for the functional characterization of the gene underlying the pleiotropic effects of qFL1 and for genetic improvement of the plant architecture and yield potential of rice.
UR - http://www.scopus.com/inward/record.url?scp=80855138102&partnerID=8YFLogxK
U2 - 10.1007/s00122-011-1669-6
DO - 10.1007/s00122-011-1669-6
M3 - Article
C2 - 21830109
AN - SCOPUS:80855138102
SN - 0040-5752
VL - 123
SP - 1319
EP - 1330
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 8
ER -