Fetomaternal trafficking in the mouse increases as delivery approaches and is highest in the maternal lung

Yutaka Fujiki, Kirby L. Johnson, Hocine Tighiouart, Inga Peter, Diana W. Bianchi

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The purpose of the study was to understand in more detail the natural history of fetomaternal cell trafficking in healthy pregnant mice. Our goal was to identify the best target organs and days during pregnancy for further mechanistic studies of the role of fetal cells in maternal disease and injury. C57BL/6J wildtype virgin females (n = 54) were mated with congenic enhanced green fluorescent protein (EGFP) transgenic males. During pregnancy and after delivery, female mice were euthanized, and eight organs and blood were analyzed for the presence of fetal GFP+ cells with flow cytometry and real-time quantitative PCR. Maternal lungs, liver, and spleen were also analyzed by fluorescent stereomicroscopy. Fetal GFP+ cells were first found at low frequency at Embryonic Day 11, increased to a maximum at Embryonic Day 19, and decreased rapidly postpartum. These fetal cell dynamics were significantly reproducible among all mice studied. In addition, there was a consistent distribution of fetal cells within maternal organs, with lung, liver, blood, and spleen having the greatest concentrations; these were highly correlated at all time points (P < 0.0001). Maternal lung contained 10- to 100-fold more fetal cells than any other organ, and using all three techniques, the number of fetal cells detected was the most consistent and reproducible in this organ. Stereomicroscopy showed that within the lung, fetal cells were widely and apparently randomly distributed. Using a murine model, our data demonstrate that fetomaternal cellular trafficking occurs in reproducible patterns, is maximal near term delivery, and has predilection for the maternal lung.

Original languageEnglish
Pages (from-to)841-848
Number of pages8
JournalBiology of Reproduction
Volume79
Issue number5
DOIs
StatePublished - Nov 2008
Externally publishedYes

Keywords

  • Fetal cell microchimerism
  • Flow cytometry
  • Lung
  • Pregnancy
  • Trafficking

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