Factors associated with humoral hypercalcemia of malignancy stimulate adenylate cyclase in osteoblastic cells

S. B. Rodan; K. L. Insogna; A. M.C. Vignery; A. F. Stewart; A. E. Broadus; S. M. D'Souza; D. R. Bertolini; G. R. Mundy

doi:10.1172/JCI111108

Factors associated with humoral hypercalcemia of malignancy stimulate adenylate cyclase in osteoblastic cells

S. B. Rodan, K. L. Insogna, A. M.C. Vignery, A. F. Stewart, A. E. Broadus, S. M. D'Souza, D. R. Bertolini, G. R. Mundy

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Abstract

The culture media of three cell lines. A human prostate carcinoma (PC3), a rat Leydig cell tumor (Rice-500), and a rat carcinosarcoma (WRC-256), that were derived from tumors associated with humoral hypercalcemia of malignancy (HHM), were examined for stimulation of adenylate cyclase in ROS 17/2.8 osteoblastic cells and for bone resorptive activity in culture. Cells from a nonhypercalcemia variant of the WRC 256 tumor served as control. Extracts from three solid human tumors, a lung adenocarcinoma from a patient with HHM and two adenocarcinomata from normocalcemia patients (lung and colon), were also examined for adenylate cyclase stimulation. We found excellent correlation between stimulation of cyclic AMP accumulation in ROS 17/2.8 cells and bone resorbing activity in culture, or production of HHM in vivo. Stimulation of adenylate cyclase by HHM factors was inhibited by the parathyroid hormone competitive inhibitor, [⁸norleucyl, ¹⁸norleucyl, ³⁴tyrosinyl] bovine parathyroid hormone (3-34) amide.

Original language	English
Pages (from-to)	1511-1515
Number of pages	5
Journal	Unknown Journal
Volume	72
Issue number	4
DOIs	https://doi.org/10.1172/JCI111108
State	Published - 1983

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title = "Factors associated with humoral hypercalcemia of malignancy stimulate adenylate cyclase in osteoblastic cells",

abstract = "The culture media of three cell lines. A human prostate carcinoma (PC3), a rat Leydig cell tumor (Rice-500), and a rat carcinosarcoma (WRC-256), that were derived from tumors associated with humoral hypercalcemia of malignancy (HHM), were examined for stimulation of adenylate cyclase in ROS 17/2.8 osteoblastic cells and for bone resorptive activity in culture. Cells from a nonhypercalcemia variant of the WRC 256 tumor served as control. Extracts from three solid human tumors, a lung adenocarcinoma from a patient with HHM and two adenocarcinomata from normocalcemia patients (lung and colon), were also examined for adenylate cyclase stimulation. We found excellent correlation between stimulation of cyclic AMP accumulation in ROS 17/2.8 cells and bone resorbing activity in culture, or production of HHM in vivo. Stimulation of adenylate cyclase by HHM factors was inhibited by the parathyroid hormone competitive inhibitor, [8norleucyl, 18norleucyl, 34tyrosinyl] bovine parathyroid hormone (3-34) amide.",

author = "Rodan, {S. B.} and Insogna, {K. L.} and Vignery, {A. M.C.} and Stewart, {A. F.} and Broadus, {A. E.} and D'Souza, {S. M.} and Bertolini, {D. R.} and Mundy, {G. R.}",

year = "1983",

doi = "10.1172/JCI111108",

language = "English",

volume = "72",

pages = "1511--1515",

journal = "Unknown Journal",

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T1 - Factors associated with humoral hypercalcemia of malignancy stimulate adenylate cyclase in osteoblastic cells

AU - Rodan, S. B.

AU - Insogna, K. L.

AU - Vignery, A. M.C.

AU - Stewart, A. F.

AU - Broadus, A. E.

AU - D'Souza, S. M.

AU - Bertolini, D. R.

AU - Mundy, G. R.

PY - 1983

Y1 - 1983

N2 - The culture media of three cell lines. A human prostate carcinoma (PC3), a rat Leydig cell tumor (Rice-500), and a rat carcinosarcoma (WRC-256), that were derived from tumors associated with humoral hypercalcemia of malignancy (HHM), were examined for stimulation of adenylate cyclase in ROS 17/2.8 osteoblastic cells and for bone resorptive activity in culture. Cells from a nonhypercalcemia variant of the WRC 256 tumor served as control. Extracts from three solid human tumors, a lung adenocarcinoma from a patient with HHM and two adenocarcinomata from normocalcemia patients (lung and colon), were also examined for adenylate cyclase stimulation. We found excellent correlation between stimulation of cyclic AMP accumulation in ROS 17/2.8 cells and bone resorbing activity in culture, or production of HHM in vivo. Stimulation of adenylate cyclase by HHM factors was inhibited by the parathyroid hormone competitive inhibitor, [8norleucyl, 18norleucyl, 34tyrosinyl] bovine parathyroid hormone (3-34) amide.

AB - The culture media of three cell lines. A human prostate carcinoma (PC3), a rat Leydig cell tumor (Rice-500), and a rat carcinosarcoma (WRC-256), that were derived from tumors associated with humoral hypercalcemia of malignancy (HHM), were examined for stimulation of adenylate cyclase in ROS 17/2.8 osteoblastic cells and for bone resorptive activity in culture. Cells from a nonhypercalcemia variant of the WRC 256 tumor served as control. Extracts from three solid human tumors, a lung adenocarcinoma from a patient with HHM and two adenocarcinomata from normocalcemia patients (lung and colon), were also examined for adenylate cyclase stimulation. We found excellent correlation between stimulation of cyclic AMP accumulation in ROS 17/2.8 cells and bone resorbing activity in culture, or production of HHM in vivo. Stimulation of adenylate cyclase by HHM factors was inhibited by the parathyroid hormone competitive inhibitor, [8norleucyl, 18norleucyl, 34tyrosinyl] bovine parathyroid hormone (3-34) amide.

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DO - 10.1172/JCI111108

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VL - 72

SP - 1511

EP - 1515

JO - Unknown Journal

JF - Unknown Journal

IS - 4

ER -

Factors associated with humoral hypercalcemia of malignancy stimulate adenylate cyclase in osteoblastic cells

Abstract

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